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EC number: 202-068-9 | CAS number: 91-44-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity study of microorganism for the test chemical
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: 2.Actived sludge 3. Tetrahymena pyriformis
- Details on inoculum:
- 2. Where possible activated sludge should be obtained from a sewage works treating predominantly domestic sewage. If this is not convenient, the activated sludge may be obtained from a sewage works treating predominantly industrial waste water. On the return to the laboratory, the sludge is centrifuged and washed with tap water. This procedure is repeated three times. A small amount of the centrifuged wet sludge is weighed and dried. After drying the ratio R of the weight of the dry solid to that of the wet centrifuged sludge is known. From this ratio R the amount of wet sludge can be calculated which has to be suspended in tap water in order to obtain an activated sludge with a mixed liquor suspended solids level of 4 g dry solids/litre. 50 ml OECD concentrate (2.2) is added to each litre of the activated sludge prepared as described above; this is then aerated overnight in a room at 20°C + 2°C, and kept aerated for use during the day. If the same sludge has to be used on subsequent days (up to a maximum of 4) a further 50 ml/litre of OECD concentrate sewage is added at the end of each working day.
3.Cultures are reared in 50 m L of a sem i -defined medium 250-m L Erlenmeyer flasks. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 40 h
- Details on test conditions:
- 2.- Test vessel: ACTIVATED SLUDGE RESPIRATION RATE APPARATUS
- Type (delete if not applicable): open / closed: No data
- Material, size, headspace, fill volume: 500 ml flask
- Aeration: Yes
- Type of flow-through: "Pasteur" pipette
-Renewal rate of test solution (frequency/flow rate): No data
- No. of organisms per vessel: No data
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates):No data
- Biomass loading rate: No data
3.Cultures are reared in 50 m L of a sem i -defined medium 250-m L Erlenmeyer flasks. - Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 40 h
- Dose descriptor:
- other: ICG50
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Study was conducted to determine effect of test chemical on micro-organism. The test chemical is showed varied value on microorganism in the concentration range of 0.18 -100 mg/l
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:
A screening method based on the measurement of the respiration rate of activated sludge for assessing the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge and the respiration rate of activated sludge with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. For those chemicals whose respiration rate is at least 80% of the control, the IC50 (concentration for 50% inhibition of respiration rate) is recorded as >100 mg/l.
Thus, IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be >100 mg/1 after 3 hrs of exposure.
The study was conducted to evaluate the toxic effects of the test chemical on the microorganism Tetrahynema pyriformis. The test was conducted for the period of 40-h in static conditions. Five different concentrations were taken to evaluate the ICG 5, median inhibition growth concentration. Test was conducted in the replicates and the growth inhibition was recorded using spectrophotometrically.Based on the results obtained from the experiment the ICG 50 was determined as 0.18 mg/L. The test chemical is showed varied value on microorganism in the concentration range of 0.18 -100 mg/l
Reference
Description of key information
Toxicity to microorganism:
Study was conducted to determine effect of test chemical on micro-organism. The test chemical is showed varied value on microorganism in the concentration range of 0.18 -100 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
Toxicity to microorganism:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:
A screening method based on the measurement of the respiration rate of activated sludge for assessing the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge and the respiration rate of activated sludge with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. For those chemicals whose respiration rate is at least 80% of the control, the IC50 (concentration for 50% inhibition of respiration rate) is recorded as >100 mg/l.
Thus, IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be >100 mg/1 after 3 hrs of exposure.
The study was conducted to evaluate the toxic effects of the test chemical on the microorganism Tetrahynema pyriformis. The test was conducted for the period of 40-h in static conditions. Five different concentrations were taken to evaluate the ICG 5, median inhibition growth concentration. Test was conducted in the replicates and the growth inhibition was recorded using spectrophotometrically.Based on the results obtained from the experiment the ICG 50 was determined as 0.18 mg/L.The test chemical is showed varied value on microorganism in the concentration range of 0.18 -100 mg/l
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