Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 237-502-6 | CAS number: 13820-53-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant, guideline study available as an unpublished report. No restrictions, fully adequate for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Diamminedichloropalladium
- EC Number:
- 238-269-3
- EC Name:
- Diamminedichloropalladium
- Cas Number:
- 14323-43-4
- Molecular formula:
- Cl2H6N2Pd
- IUPAC Name:
- Diamminedichloropalladium
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Diamminedichloropalladium
- Molecular formula (if other than submission substance): C12H6N2Pd
- Molecular weight (if other than submission substance): 211.39 g/mol
- Physical state: Yellow powder
- Analytical purity: >99.5%
- Composition of test material, percentage of components: 50.33% palladium
- Lot/batch No.: 11011
- Expiration date of the lot/batch: 31 October 2012
- Storage condition of test material: Closed dry vessel at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant D-67435 NW-Lachen-Speyerdorf and aerated during transport. Upon arrival at the test facility the sludge was filtered, washed with tap water and resuspended in tap water. The activated sludge was aerated until usage and fed daily with 50mL/L synthetic sewage feed.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 1.08 mmol/L
- Test temperature:
- Pre-test: 19.1 - 20.8°C
Main study: 19.7 - 20.8°C - pH:
- Pre-test: 6.25 - 8.25 at end of test
Main study: 7.6 - 8.0 at end of test - Dissolved oxygen:
- Not reported
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Pre-test: Nominal Control, 1, 10, 100 and 100 mg/L
Main study: Nominal Control, 10, 20, 30, 60, 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: Glass containing 500 mL test solution, nutrient solution and inoculum
- Aeration: purified air through pasteur pipette at 0.75L/min flow rate
- No. of vessels per concentration (replicates): Pre-test: 1 per test item concentration except highest contentration (5 replicates); Main study: 5 per test item concentration
- No. of vessels per control (replicates): Pre-test: 6 Control, 4 positive control; Main study: 6 Control, 4 positive control
- Biomass loading rate: Pre-test: 1.08g suspended solids/L; Main-study: 1.35 g suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Range finding study: yes
- Test concentrations: 0, 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: -4.2% inhibition at 1 mg/L, -0.6% inhibition at 10 mg/L, 81.7% inhibition at 100 mg/L, 100% inhibition at 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Diamminedichloropalladium
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL 28 - 30 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 61 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Diamminedichloropalladium
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL 59 - 63 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Diamminedichloropalladium
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14 590 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- 3-hour EC50 8.8 mg/L (95% CL 5.0 - 13 mg/L) which lies within the recommended range of 2- 25 mg/L.
- Reported statistics and error estimates:
- Oxygen consumption was calculated using linear regression.
EC50 was calculated using linear fit on a probability-logarithmic scale using Origin™ software.
NOEC was determined by testing equality of variance using and t-test or WEIR test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC10 is 29 mg DDP/L (95% CL 28 - 30 mg/L). The 3-hour EC50 is 61 mg DDP/L (95% CL 59 - 63 mg/L). The NOEC is 18 mg DDP/L. This corresponds to 14.6 mg Pd L-1, 30.7 mg Pd L-1 and 9.1 mg Pd L-1, respectively.
- Executive summary:
A GLP compliant, OECD guideline study to assess the inhibition of respiration of activated sludge was carried out (LAUS 2012). The study is considered to be suitable for use as the key study for this endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.