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EC number: 248-053-0 | CAS number: 26850-47-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-[[4-[ethyl(2-hydroxyethyl)amino]phenyl]azo]-6-methoxy-3-methylbenzothiazolium chloride
- EC Number:
- 248-053-0
- EC Name:
- 2-[[4-[ethyl(2-hydroxyethyl)amino]phenyl]azo]-6-methoxy-3-methylbenzothiazolium chloride
- Cas Number:
- 26850-47-5
- Molecular formula:
- C19H23ClN4O2S
- IUPAC Name:
- 2-[(E)-2-{4-[ethyl(2-hydroxyethyl)amino]phenyl}diazen-1-yl]-6-methoxy-3-methyl-1,3-benzothiazol-3-ium chloride
- Test material form:
- solid
- Details on test material:
- see below
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif:RAIf(SPF)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CIBA-GEIGY LTD. Tierfarm, 4334 Sisseln, Switzerland
- Females (if applicable) nulliparous and non-pregnant: [yes]
- Age at study initiation: 7-8 weeks
- Weight at study initiation: 157-190 g
- Fasting period before study:
- Housing: 5/cage/sex
- Diet (e.g. ad libitum): NAFAG No. 890, NAFAG AG, Gossau, SG (Switzerland) ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 55 +/- 15
- Air changes (per hr): ca. 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: March 3, 1982 To: March 29, 1982
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- 400
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 and 20 mL/kg
MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg - Doses:
- 300, 1000, 2500, 5000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Mortality: daily, a.m. and p.m. on working days
Signs and Symptoms: daily
Body weight: on days 1, 7, 14 and at death
- Necropsy of survivors performed: yes
Necropsies: Spontaneously dying animals were submitted to a gross necropsy as soon as possible; survivors at the end of the observation period.
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: - Statistics:
- From the body weights, the group means and their standard deviations were calculated.
The LD50 including the 95% confidence limit were computed by the logit method (J. Berkson, J.Am. Stat. Ass. 39.357-65, 1944)
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 004 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 966 - <= 7 827
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 152 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 660 - <= 82 457
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 120 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 1 279 - <= 4 373
- Mortality:
- No deaths occurred at 300 mg/kg bw.
At 1000 mg/kg bw, 1 male and 2 females died within two days.
At 2500 mg/kg bw, 2 males and 2 females died within two days.
At 5000 mg/kg bw, all males and 3 females died on Day 1; one further female died on Day 13. - Clinical signs:
- Only signs of unspecific toxicity were observed at all dose levels, such as, sedation, dyspnoea, exaphthalmos, ruffled fur, curved body position
- Body weight:
- All surviving animals showed regular body weight development
- Gross pathology:
- No compound related gross organ changes were observed.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- calculated for 100% API
- Executive summary:
Upon a acute oral administration and a 14 day post-treatment observation period, the following lethal doses (with 95 % confidence limits) were
determined for Basic Blue 41 Cl (purity 55.6%).
LD50 in male rats: 2004 (966-7827) mg/kg bw.
LD50 in female rats: 2152 (660-82457) mg/kg bw.
LD50 in rats of both sexes: 2120(1279-4373) mg/kg bw.
Signs of unspecitic toxicity were seen at all dose levels.
According to the company standard Basic Blue 41 has a slight acute toxicity when administered orally to the albino rat.
Calculated for 100% API, the LD50 lies between 300 and 2000 mg/kg bw, and hence results in classification in Category 4 based on GHS CLP criteria.
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