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EC number: 700-509-4 | CAS number: 19294-29-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- Potassium 4-methylbenzenesulfinate
- EC Number:
- 700-509-4
- Cas Number:
- 19294-29-2
- Molecular formula:
- C7H7KO2S
- IUPAC Name:
- Potassium 4-methylbenzenesulfinate
- Details on test material:
- - Name of test material (as cited in study report): CGA 311117 tech.
- Physical state: solid
- Purity: 98.5%
- Lot/batch No.: V.24-30-10
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Type and identity of media:
Without metabolic activation:
Strain Mutagen Solvent
TA 100 sodium azide distilled water
TA 1535 sodium azide distilled water
TA 98 2-nitrofluorene DMSO
TA 1537 9 (5)-aminoacridine DMSO
With metabolic activation:
Strain Mutagen Solvent
TA 100 2-aminoanthracene DMSO
TA 1535 cyclophosphamide*H2O bidistilled water
TA 98 2-aminoanthracene DMSO
TA 1537 2-aminoanthracene DMSO
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- - Type and identity of media:
Without metabolic activation:
Strain Mutagen Solvent
WP2 uvrA 4-nitroquinoline-N-oxide DMSO
With metabolic activation:
Strain Mutagen Solvent
WP2 uvrA 2-aminoanthracene DMSO
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Range in cytotoxicity test: 20.6 - 5000µg/plate
Range in mutagenicity test:
Without metabolic activation: 312.5-5000µg/plate
With metabolic activation:: 312.5-5000µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: bidistilled water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- solvent alone
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: without metabolic activation for TA 100 and TA 1535
- Untreated negative controls:
- yes
- Remarks:
- sovent alone
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- Migrated to IUCLID6: without metabolic activation for WP2 uvrA
- Untreated negative controls:
- yes
- Remarks:
- solvent alone
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Migrated to IUCLID6: without metabolic activation for TA 98
- Untreated negative controls:
- yes
- Remarks:
- solvent alone
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: without metabolic activation for TA 1537
- Untreated negative controls:
- yes
- Remarks:
- solvent alone
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene: with metabolic activation for TA 100, WP2 uvrA, TA 98 and TA 1537
- Untreated negative controls:
- yes
- Remarks:
- solvent alone
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- Migrated to IUCLID6: with metabolic activation for TA 1535
- Details on test system and experimental conditions:
- Control of genotype:
- Characteristics of the strains were checked monthly
Method of application: in agar (plate incorporation)
Metabolic activation mixture:
Rat liver S9 fraction (100µl/ml), NADP (4µmol/ml), MgCl2 (8µmol/ml), KCl (33µmol/ml), Na-phosphate buffer pH 7.4 (100µmol/ml), glucose-6-phosphate (5µmol/ml)
Duration:
- Incubation period: 48 hr at 37 ± 1.5°C inverted in darkness
Assay acceptance criteria:
A test is considered acceptable if the mean colony counts of the negative control values of all strains are within the acceptable ranges and if the results of the positive controls meet the criteria for a positive response. - Evaluation criteria:
- Criteria for a positive response - the substance was deemed to be mutagenic in this test if one or both of the following conditions were met:
- at least a reproducible doubling of the mean number of revertants per plate above that of the negative control at any concentration for one or more of the strains
- a reproducible increase of the mean number of revertants per plate for any concentration above that of the negative control by at least a factor of 1.5 for strain S.typhimurium TA 100 - Statistics:
- A statistical analysis was not performed as the use of statistical methods is not generally recommended
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Based upon the results of these experiments it can be concluded that this substance and it's metabolites did not induce gene mutations in the strains of S.Typhimurium and E.coli used.
- Executive summary:
The experiments were performed with and without metabolic activation. Normal background growth was observed in both strains.
None of the tested concentrations of CGA 311117 tech. led to an increase in the incidence of either histidine- or tryptophan- prototrophic mutants by comparison with the negative control.
There were no known circumstances or occurrances in this study that were considered to have affected the quality or integrity of the data.
Based upon the results of these experiments there is no evidence to suggset this substance should be classed as either cytotoxic nor mutagenic.
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