Reaction mass of (2S,6S)-2,6-Dimethylmorpholine and (2R,6R)-2,6-Dimethylmorpholine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication/ study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

BASF-Test, see details in remarks on material and methods

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): 2,6-Dimethyl-morpholin (75 % cis/25 % trans)
- Physical state: liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: ca. 50 cm2

REMOVAL OF TEST SUBSTANCE
- Washing (if done): water/Lutrol (1:1)
- Time after start of exposure: 24 h

Duration of exposure:

14 days

Doses:

1000, 2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: several days on the day of application and daily thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 - < 2 000 mg/kg bw

Mortality:

see details in table in remarks on results.

Clinical signs:

Sytemic symptoms:
dyspnoea, apathy, staggering, tremor, spastic gait, ruffled fur, diarrhea, cyanosis, exsiccosis, eyelid closure, poor general state.

Local symptoms:
After 24 h: severe soft necrosis, slight edema,
After 7 days: severe parchment-like necrosis, slight edema,
After 14 days: severe parchment-like to leathery necrosis.

Body weight:

no data

Gross pathology:

Animals that died:
Heart: acute dilatation of the ventricle; acute congestion;
Stomach: bloody ulcerations;
Liver: grey-colored lobular periphery;

Any other information on results incl. tables

Mortality:

Dose mg/kg	Conc. %	Animal	1 h	24 h	48 h	7 days	14 days
2000	100	3 m 3 f	0/3 0/3	2/3 3/3	3/3 3/3	3/3 3/3	3/3 3/3
1000	100	3 m 3 f	0/3 0/3	0/3 0/3	0/3 1/3	0/3 1/3	0/3 1/3