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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Version / remarks:
Directive 79/831/EEC, Annex V, CY, Part IV, Manometric Respirometry
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
The activated sludge inoculum used in this study originated from laboratory wastewater treatment plants fed with municipal sewage. The concentration of dry substance was 30 mg/L.
Duration of test (contact time):
28 d
Initial conc.:
100.8 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
0 - 10
Sampling time:
28 d
Details on results:
Abiotic processes in a batch without inoculum:
O2*100/COD = 2 %
Parameter:
BOD5
Value:
0 mg O2/g test mat.
Parameter:
COD
Value:
2 221 mg O2/g test mat.
Parameter:
ThOD
Value:
2 024 mg O2/g test mat.
Results with reference substance:
Kinetic of control substance (in %):
= 63 after 9 day(s)
The inoculum was not significantly inhibited by the test substance- Abiotic processes in a batch without inoculum: 2 % Pretests:
---------
Elementary analysis C = 79.5% C
Interpretation of results:
under test conditions no biodegradation observed

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information