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EC number: 200-665-9 | CAS number: 67-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from peer-reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of Genotoxicity on Plant-Derived Dietary Sulfur
- Author:
- LEE, YOON-IK
- Year:
- 2 006
- Bibliographic source:
- J. Microbial. Biotechnol.
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate genetic potential of substance dimethyl sulphone (Methylsulfonylmethane).
- GLP compliance:
- no
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Dimethyl sulphone
- EC Number:
- 200-665-9
- EC Name:
- Dimethyl sulphone
- Cas Number:
- 67-71-0
- Molecular formula:
- C2H6O2S
- IUPAC Name:
- dimethyl sulphone
Constituent 1
Method
- Target gene:
- Chinese Hamster Lung (CHL) cells
Species / strain
- Species / strain / cell type:
- mammalian cell line, other: Chinese Hamster Lung (CHL) cells
- Details on mammalian cell type (if applicable):
- Details on mammalian cell line
- Type and identity of media: The cell were cultured in Eagles minimum essential medium (EMEM) supplemented with 10% fetal bovine serum. The cell were incubated in a 95% air and 5% CO₂ atmosphere at 37°C. The cell were plated at a density of 2.5ẋ10⁵ cells on 6cm plates and grown for 22h, and then colcemid (0.25g/ml) was added 2hr before harvest.
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: No data available
- Periodically checked for karyotype stability: No data available
- Periodically "cleansed" against high spontaneous background: No data available - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 fraction
- Test concentrations with justification for top dose:
- 0, 5, 2.5, 1.25 mg/ml
- Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: No data available
- Justification for choice of solvent/vehicle: No data available
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- methylmethanesulfonate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: plate incorporation
DURATION
- Preincubation period: No data available
- Exposure duration: 24hr
- Expression time (cells in growth medium): 22 hr
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): 24 hr
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: No data available
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: No data available
OTHER EXAMINATIONS
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available - Rationale for test conditions:
- No data
- Evaluation criteria:
- No data
- Statistics:
- No data
Results and discussion
Test results
- Key result
- Species / strain:
- mammalian cell line, other: Chinese Hamster Lung (CHL) cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data available
- Effects of osmolality: No data available
- Evaporation from medium: No data available
- Water solubility: No data available
- Precipitation: No data available
- Other confounding effects: No data available
RANGE-FINDING/SCREENING STUDIES: No data available
COMPARISON WITH HISTORICAL CONTROL DATA: No data available
ADDITIONAL INFORMATION ON CYTOTOXICITY: No data available - Remarks on result:
- other: no mutagenic potential
Applicant's summary and conclusion
- Conclusions:
- In the in vitro chromosome aberration test using Chinese Hamster Lung (CHL) cells, no abberation effects were seen.Therefore, the test substance is considered to be non mutagenic in Chinese Hamster Lung (CHL) cells.
- Executive summary:
The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate the potential of test substance damaging chromosome. The cell were exposed to test substance at concentration 0, 5, 2.5, 1.25 mg/ml for 24 hr. Methylmethane sulfon ate (MMS)(0.02 mg/ml) and Benzo[a]pyrene (B[a]P) (0.02mg/ml) used as positive control substances. After treatment with test substance , no significant increase in the number of aberrant cells was observed. In presence or absence of S9 mix, the chromosome aberration produced by treatment with Methylsulfon ylmethane was less than 2 %. These results indicate that methylsulfonylmethane did not increase chromosome aberration as compared with the negative control. Therefore, the test substance is considered to be non genotoxic.
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