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Diss Factsheets
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EC number: 224-081-9 | CAS number: 4196-89-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without restriction
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- SAMPLING SCHEDULE:
- Control: at 0 h (2014-09-01, 2014-09-02, 2014-09-03, 2014-09-04) and 24 h (2014-09-02, 2014-09-03, 2014-09-04 and 2014-09-05) according to the semi-static conditions
- Test concentrations : 100 mg/L in the freshly prepared media (on 2014-09-01, 2014-09-02, 2014-09-03 and 2014-09-04) and in the media after 24 h of exposure (on 2014-09-02, 2014-09-03, 2014-09-04 and 2014-09-05) according to the semi-static conditions - Vehicle:
- no
- Details on test solutions:
- Pre-TREATMENT OF TEST ITEM AND PREPARATION OF TEST ITEM CONCENTRATIONS:
- Direct weighings were prepared daily to produce the test item concentration.
- To achieve this 500 mg of the test item were added daily to 5 litres of dilution water, treated for 1 h in an ultrasonic bath and stirred for 3 h on a magnetic stirrer.
- Undissolved particles of the test item were removed by filtration using a folded filter with a pore size of 7-12 μm.
- The pH was adjusted to pH 7.0 to reduce hydrolytic processes. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST SPECIES:
- Name: Zebra fish (Danio rerio)
- Source: West Aquarium GmbH (Germany)
- Date supplied: 2014-06-12
- Acclimatisation: Stock held since 2014-06-12 and acclimatised to the test conditions since then
MAINTANANCE:
- Temperature: 20-24°C
- Dissolved oxygen: > 5 mg/L
- Feeding : Commercial fish food, daily; feeding discontinued 24 h prior to test start
- Holding water: dechlorinated tap water: hardness 15° dH, acidity: 0.1 +/- 0.02 mmol/l; Ca/Mg: 4:1; pH: 6.3-6.8; oxygen: >= 90 % of saturation
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 14): 3.58 cm (S.D. = 0.22 cm) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 14.0 °dH (250 mg/L CaCO3)
- Test temperature:
- 21.5 to 22.1 °C
- pH:
- adjusted to pH 7 after day 1, 2 and 3
- Dissolved oxygen:
- 7.8 - 9.1 (mg/L)
- Salinity:
- n.a.
- Nominal and measured concentrations:
- Effective concentrations ranged from 0.33 % to 0.74 % of nominal values in the freshly medium and from 0.08 % to 0.36 % of nominal values in the medium after 24 hours of exposure.
- Details on test conditions:
- EXPOSURE CONDITIONS:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 1 test concentration plus 1 control; 7 fish per test concentration; no feeding during the exposure period
- Method of initiation: fish were placed in prepared media
- Loading: 0.95 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration/s: 100 mg/L
- Method of administration: direct weighing
- Medium renewal: daily
- Criteria of effects: The criterion of death was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed. - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.28 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.158 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of geometric mean measured concentrations.
- Validity criteria fulfilled:
- yes
- Remarks:
- (mortality in the controls < 10% by the end of the test; dissolved oxygen concentration above 60% of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit)
- Conclusions:
- The toxicity of 2,2-dimethylpropane-1,3-diyl dibenzoate to fish was determined at LC50 of higher than 0.28 mg/L after 96 hours. No toxic effects against fish were observed at the limit of water solubility under exposure conditions. All results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 0.33 % to 0.74 % of nominal values in the freshly medium and from 0.08% to 0.36 % of nominal values in the medium after 24 hours of exposure.
- Executive summary:
A study was performed to assess the acute toxicity of 2,2-dimethylpropan-1,3-diyl dibenzoate to Danio rerio under semi-static conditions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 100 mg/L of 2,2-dimethylpropan-1,3-diyl dibenzoate dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and folded filters. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure.
No toxic effects of 2,2-dimethylpropane-1,3-diyl dibenzoate against fish were observed at the limit of water solubility under exposure conditions. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Fish study.
Reference
Description of key information
The toxicity of 2,2-dimethylpropane-1,3-diyl dibenzoate to fish was determined at LC50 of higher than 0.28 mg/L after 96 hours. No toxic effects against fish were observed at the limit of water solubility under exposure conditions. All results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 0.33 % to 0.74 % of nominal values in the freshly medium and from 0.08% to 0.36 % of nominal values in the medium after 24 hours of exposure.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.28 mg/L
Additional information
Should read: ">0.28 mg/L"
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