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EC number: 203-104-6 | CAS number: 103-36-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Restriction: No strain included that could detect certain oxidising or cross-linking agents (e.g. TA 102); no information on positive control substances
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- The method of Ames, McCann & Yamasaki (1975) was followed.
- Deviations:
- yes
- Remarks:
- Deviation from OECD TG 471: No strain included that could detect certain oxidising or cross-linking agents (e.g. TA 102)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl cinnamate
- EC Number:
- 203-104-6
- EC Name:
- Ethyl cinnamate
- Cas Number:
- 103-36-6
- Molecular formula:
- C11H12O2
- IUPAC Name:
- .
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA92
- Details on mammalian cell type (if applicable):
- The test strain was originally provided by Dr B. N. Ames, University of California, Berkeley, USA.
- Species / strain / cell type:
- S. typhimurium, other: TA94
- Details on mammalian cell type (if applicable):
- The test strain was originally provided by Dr B. N. Ames, University of California, Berkeley, USA.
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- The test strains were originally provided by Dr B. N. Ames, University of California, Berkeley, USA.
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver microsome fraction (S-9) prepared from the liver of Fischer rats (Charles River Japan Co.)
- Test concentrations with justification for top dose:
- Six concentrations were tested (max. 5.0 mg/plate) to determine the maximum dose.
- Vehicle / solvent:
- dimethylsulphoxide (DMSO)
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation with the test sample and with or without the S-9 mix
DURATION Preincubation: 20 min at 37°C
NUMBER OF REPLICATIONS: 2 for each concentration
OTHER: If no reasonable dose response was detected, additional experiments using different doses or induced mutation frequency assays were performed. - Evaluation criteria:
- The number of revertant (his+) colonies was scored after incubation at 37°C for 2 days.
The result was considered positive if the number of colonies found was twice the number in the control (exposed to the appropriate solvent or untreated).
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA94
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA92
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Additional information on results:
- The maximum dose was 5.0 mg/plate, which represents the highest non-cytotoxic dose used in the experiment.
Applicant's summary and conclusion
- Conclusions:
- The test item was found not to be genotoxic in an Ames test using the S. typhimurium strains TA92, TA1535, TA100, TA1537, TA94 and TA98.
- Executive summary:
In the current study no OECD guideline was followed and the study was not according to GLP, however, the method of Ames, McCann & Yamasaki (1975) was followed which is similar to the OECD Guideline 471.
The assay included the S. typhimurium strains TA92, TA1535, TA100, TA1537, TA94 and TA98 and the genotoxicity of the test item was investigated with and without metabolic activation, which was the liver microsome fraction (S-9) from Fischer rats.
The test item was found to be negative for all S. typhimurium strains, which indicates that no significant increase in the number of revertant colonies were detected at a maximum dose of 5.0 mg/plate, at which no cytotoxic effects were observed.
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