Fatty acids, C18 (saturated and unsaturated) ethyl esters

Endpoint summary

Additional information

The test material is reported to be a pale yellow liquid at room temperature. The test material is a mixture of different chemical species. The measured melting temperature vary between -9 °C and up to 19 °C. The peak value is 14.7 °C. The substance is a mixture of different substances with different boiling points. The test item has been determined to boil over the range of approximately 225 to 396 °C (498 to 669 K) at 101 kPa using differential scanning calorimetry. The peak value was between 363 and 364 °C.

The density of the test item has been determined to be 881 kg/m3 at 20.0 ± 0.5 °C, with a relative density of 0.881 using the pycnometer method in accordance with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 02 October 2012.

Granulometry study is scientifically unjustified as adaption to column 2 (7.14): The substance is liquid at room temperature. The vapour pressure was measured using a vapour pressure balance method in accordance with OECD 104. Four vapour pressure-temperature correlations were estabilshed for the temperature range 65-75 °C, and the vapor pressure at 25 °C was estabilshed by extrapolating each correlation to 25 °C, and using the average value. The result is 0.0011 Pa at 25 °C.

Partition Coefficient was determined using the HPLC method in accordance with Method A8 Partition Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 117 of the OECD Guidelines for Testing of Chemicals, 13 April 2004. The partition coefficient has been determined to be : log10(Pow) of >6.50.

The water solubility was carried out by the flask method in accordance with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995. A preliminary test and a main test (3 samples) was carried out. The preliminary estimate of water solubility was less than 0.1 mg/L at 20.0 °C. The concentration (mg/L) of the substance in the sample solutions were also all below 0.1 mg/L at 20 °C. Thus, the substance is concluded to be insoluble.

A study on surface tension does not need to be carried out, as the substance has a water solubility below 1 mg/L (Annex VII, 7.6, column II).

Autoignition temperature was determined to be 248 (+/- 5) °C at ambient pressure of 101.5-102.2 kPa and room temperature (20-21 °C).

The substance is a mixture of fatty acids and fatty acids alkyl esters. It has a very low water solubilty and will not form any flammable gases or vapors upon contact with water. When mixed with water it will readily form a layer above the water layer containing almost no water. The substance itself has a high flash point (189 °C) and an autoignition temperature of 248 °C, so it does not react with air at normal conditions.

Based on predictions using a procedure compatible with Method A14 Explosive Properties of Commission Regulation (EC) No 440/2008 of 30 May 2008. The substance is a mixture of fatty acids and fatty acids alkyl esters and does not contain any chemical groups that imply explosive properties.

The substance is an organic incapable of reacting exothermically with combustible materials. Based on the chemical structure of the test item, using a procedure designed to be compatible with Method A21 Oxidising Properties (Liquids) of Commission Regulation (EC) No 440/2008 of 30 May 2008.

The stability of the substance in hexane in darkness was tested at 25 ± 1 °C for a period of 30 days. Sample solutions were analysed at various times, and the concentration of substance in the sample solutions was determined by high performance liquid chromatography – mass spectrometry (HPLC-MS). The substance appears to be stable in the tested organic solvent (hexane).

The dissociation constants in water was not determined as the substance is a complex mixture that is insoluble in water and as the majority of the components do not have a dissociation constant within the environmentally relevant pH range (organic esters).

The viscosity was determined using a capillary viscometer method in accordance with Method 114 of the OECD Guidelines for Testing of Chemicals, 02 October 2012. The results are as follows: Viscosity at 20.0 ± 0.5 °C: 11.5 mm2/s; viscosity at 40.0 ± 0.5 °C: 6.78 mm2/s.