Benzoic acid, 2,3,4,5-tetrachloro-6-cyano-, methyl ester, reaction products with 2-methyl-1,3-benzenediamine and sodium methoxide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1970

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP non-guideline study, available as unpublished report, predates implementation of GLP and/or development of study guidelines, restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

The test substance is administered orally by gavage at a dose level of 5000 mg/kg to rats of both sexes. Test substance was in suspension with arabic gum (1%)/ tap water. Application was followed by a 8 day observation period.

GLP compliance:

no

Remarks:

prior to GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Mixture of octachloro, monomethoxy-heptachloro and bismethoxy-hexachloro derivatives of 3,3'-[(2-methyl-1,3-phenylene)diimino]bis[2,3-dihydro-1H-isoindol-1-one]

EC Number:

600-735-2

Cas Number:

106276-79-3

Molecular formula:

Mixture of C23H8Cl8N4O2, C24H11Cl7N4O3, C25H14Cl6N4O4

IUPAC Name:

Mixture of octachloro, monomethoxy-heptachloro and bismethoxy-hexachloro derivatives of 3,3'-[(2-methyl-1,3-phenylene)diimino]bis[2,3-dihydro-1H-isoindol-1-one]

Test animals

Species:

rat

Strain:

other: CFE (SPF)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 5- 6 weeks
- Weight at study initiation: average 135 g
- Housing: 5 animals per cage
- Diet: Nafag Nr. 185
- Maintenance: in air-conditioned room
- Fasting period before study: yes

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Arabic gum (1%)/ tap water

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg

Application concentration: 25%

DOSAGE PREPARATION (if unusual):
Fresh suspension with Arabic gum (1%)/ tap water

Doses:

5000 mg/kg

No. of animals per sex per dose:

5 animals/dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 8 days
- Frequency of weighing: at start and end of study period
- Symptoms recorded: yes

Statistics:

Interpolation on Probability (Schleicher and Schüll No. 298 ½)

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

No animals died.

Clinical signs:

other: Following symptoms were recorded: somnolence dyspnea exophthalmos ataxia yellow discolored feces

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met