Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 239-263-3 | CAS number: 15206-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany.
The inoculum was collected on 22 October 2014 from the aeration tank of the plant. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 24 hours at 22 ± 2° C.
At the day of exposure the suspension was washed one time with drinking water. Subsequently the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with drinking water and the concentration of the sludge was adjusted to 6.0 g/L dry weight.
Aliquots of 7.5 mL were added to the test vessels to obtain an activated sludge concentration of 30 mg/L dry weight. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 31 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
The used mineral medium complies with the test guideline OECD 301B. It was prepared as follows:
Solution A: KH2PO4 : 8.50 g
K2HPO4 : 21.75 g
Na2HPO4 × 2 H2O : 33.40 g
NH4Cl : 0.50 g
The compounds were dissolved with deionized water to 1000 mL; the pH value was adjusted to 7.4.
Solution B: CaCl2 × 2 H2O : 36.40 g
The compound was dissolved with deionized water to 1000 mL
Solution C: MgSO4 × 7 H2O : 22.50 g
The compound was dissolved with deionized water to 1000 mL
Solution D: FeCl3 × 6 H2O : 0.25 g
The compound was dissolved with deionized water to 1000 mL
15 mL solution A, 1.5 mL solution B, 1.5 mL solution C and 1.5 mL solution D was used for the preparation of the test assays.
- Test temperature: 22 ± 2° C
- pH: 7.4 ± 0.2,
- pH adjusted: yes (if necessary)
- Aeration: At begin of the exposure phase the test vessels were connected with an aeration unit and the bubble aeration with carbon dioxide free air was started after connecting the several test vessels with the absorption units.
TEST SYSTEM
- Culturing apparatus: 2 L incubation bottles filled up to a volume of 1.5 L
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions:
- Measuring equipment: The TIC- and DOC-analyses were performed as repeat determination, using a TOCanalyzer equipped with an auto sampler (Shimadzu TOC-5000A and/or TOC-L CSH/CSN). The system works with a combustion/non-disperse infrared gas analysis method. For calibration of the TOC-Analyzer, standard samples were measured before start of measurements to prove the conformity with the calibration curve. The samples for TICanalysis (absorption solution) were measured without further treatment.
The samples for the DOC-analysis were centrifuged for about 15 minutes at 4000 rpm. The samples were analyzed on the day of sampling.
-Experimental procedure: The Carbon Dioxide Evolution Test was performed in 2 L incubation bottles filled up to a volume of 1.5 L. The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. Usually twice a week the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined and used for the calculation of the produced carbon dioxide. After each sampling the second trap was moved forward and the new trap with fresh sodium hydroxide solution was placed into the second position. Each trap was analyzed separately.
The TIC-value of the freshly prepared sodium hydroxide solution was determined and considered by the calculation of biogenic produced carbon dioxide amount. The incubation bottles were stirred on magnetic stirrers; the aeration was performed with carbon dioxide free air at a flow of approximately 800 mL per hour.
At the end of exposure, the pH values were measured in each test vessel. For stripping of carbon dioxide, dissolved in the test medium, each test vessel was acidified by adding 2 mL of concentrated hydrochloric acid. The concentration of dissolved organic carbon in the blank controls and reference substance assays were determined. Since the test substance was sufficiently soluble in water, DOC measurements could be also performed from the inhibition control and test substance assays.
The aeration was continued for about 24 hours and the released carbon dioxide amounts in both traps of each test vessel were determined and added to the calculated amount of the previous day. - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 90 - 100
- Sampling time:
- 28 d
- Details on results:
- The required pass level for ready biodegradability within a ten days window was reached. The degree of biodegradation at the end of the ten day window was approx. 82% CO2/ThCO2.
Degree of DOC removal of test substance at the end of exposure: 90-100 %
Degree of biodegradation of the test substance at the end of exposure, mean value: 90-100 % CO2/ThCO2 - Results with reference substance:
- Degree of biodegradation of the reference substance (aniline) after 14 days: 92 % CO2/ThCO2
Degree of biodegradation in the inhibition control after 14 days: 79 % CO2/ThCO2 - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance is readily biodegradable according OECD criteria.
- Executive summary:
In this guideline (OECD 301 B) study, the test material (EC 239-263-3) was determined to be "readily biodegradable". The test material achieved 82% degradation (CO2/ThCO2) at 10 days, and 90 -100% degradation (CO2/ThCO2) at 28 days. The test material was determined to meet the criteria to be considered "Readily biodegradable" in accordance with the OECD 301 guideline.
Reference
Description of key information
Study carried out according to guideline and with GLP compliance.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A GLP guideline study according to OECD 301B has been conducted (BASF SE 2015). After 28 d of exposure 90 - 100 % biodegradation was observed in the carbon dioxide evolution test based on the quantitative determination of the formed carbon dioxide in the test substance assays by comparison with the calculated maximal theoretical carbon dioxide production. Since the required pass level for ready biodegradability within a ten days window was reached the test substance is regarded as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.