Disodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxybenzenesulphonato(3-)][1-[[2-hydroxy-5-(phenylazo)phenyl]azo]-2-naphtholato(2-)]chromate(2-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

Method A-11

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 21095/B
Purity: 86.1 %

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- 10 per test concentration
- Age: about 4-6 months
- Size: about 3 cm
- Weight: 0.25-0.35 g
- Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg 
-  Acclimatization period: minimum 12 days at 21-25 °C for Zebra fish.
-  Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
-  Day-night cycles: 12/12 h
-  Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

25 °C

pH:

8 - 8.2

Dissolved oxygen:

6.8 - 7.7

Nominal and measured concentrations:

Nominal concentration: 1, 3, 10 and 30 mg/L.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

other: TL0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL50

Effect conc.:

3.11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 1 mg/L: 10/10 fishes survived at 96h
At 3 mg/L; 7/10 fishes survived at 96h.
At 10 mg/L: 0/10 fishes survived at 4h interval.
At 30 mg/L: 0/10 fishes survived at 1h interval.

Validity criteria fulfilled:

not specified

Conclusions:

The TL50 in the acute fish toxicity test was determined to be 3.83 mg/l at 48 hours and 3.11 mg/l after 96 hours exposure time.

Executive summary:

An acute fish toxicity test performed following a internal method equivalent or similar to OECD test guideline 203 using Zebra fish. Fish were exposed to test substance FAT 21095/B for 96 hr to determine the TL values at following concentrations; 1, 3, 10 and 30 mg/L. The following 96h results were obtained as follows:

TL0: 1 mg/L

TL50: 3.11 mg/L

TL100: 10 mg/L

Description of key information

An acute fish toxicity test performed following a guideline equivalent or similar to OECD test guideline 203 using Zebra fish. Fish were exposed to FAT 21095 for 96 h to determine the TL values. The TL50 in the acute fish toxicity test was determined to be 3.83 mg/l at 48 hours exposure time and 3.11 mg/l after 96 hours exposure time. Considering the results of key study, FAT 21095 is considered to be toxic to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.11 mg/L