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EC number: 204-679-6 | CAS number: 124-09-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The test item had no adverse effects on N-transformation (OECD 216; GLP; RL1; 2017) in lucerne amended soil during 28 days at test item concentrations of up to and including 1000 mg/kg soil dry weight. Due to the lacking of adverse effects inhibitory concentrations (ICx) could not be calculated. Consequently, ICx are considered to be higher than 1000 mg/kg soil dry weight which was the highest tested concentration. Accordingly, LOAEC and NOAEC are > and ≥ 1000 mg/kg soil dry weight, respectively.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
A valid nitrogen transformation test was performed with the submission substance in 2017 according to OECD 216, compliant with GLP (RL 1).
Five test item concentrations were tested against an untreated control (C) with four replicates, each: 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight. The soil test was amended with ground lucerne grass green meal (5.0 g/kg soil dw) except for treatment TC which consisted of soil without lucerne and was treated with the test item at 250 mg/kg soil dw. Nitrate of the soil was measured after test item application on day 0, and 28 days after test item application.
The following results were obtained for the submission substance:
Mean nitrate concentration at test start (day 0) ranged from 57.6 to 59.6 mg/kg soil dry weight (control and test item treatments T1 to T5). Mean nitrate concentration for soil without lucerne was 43.7 mg/kg soil dw (bulk soil) and 43.3 mg/kg soil dw (treatment TC).
Nitrate concentration in soil increased considerably in all test soils from day 0 to day 28 thus confirming the existence of a metabolically active microbial biomass in the soil.
Mean nitrate concentration on day 28 had increased to 112.8 mg/kg soil dw (control). In the test item treated soils it ranged from 149.4 mg/kg soil dw to 698.6 mg/kg soil dw (206.9 mg/kg soil dw in treatment TC).
Nitrate concentration in soil on day 28 increased with the test item concentration. This observation strongly suggests that nitrogen from the test item was transformed to nitrate by the soil microorganisms.
No adverse effects of the test item on nitrate formation were observed at any of the test item concentrations up to and including 1000 mg/kg soil dw which was the highest tested concentration.
Due to the lack of any adverse effects, inhibitory concentrations (ICx) could not be determined. Consequently, IC50, IC20 and IC10 can be considered to be higher than 1000 mg/kg soil dry weight. Accordingly, LOAEC and NOAEC are >1000 mg/kg soil dw and ≥1000 mg/kg soil dw, respectively.
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