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EC number: 224-330-1 | CAS number: 4314-14-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,4-dihydro-5-methyl-2-phenyl-4-(phenylazo)-3H-pyrazol-3-one
- EC Number:
- 224-330-1
- Cas Number:
- 4314-14-1
- Molecular formula:
- C16H14N4O
- IUPAC Name:
- 2,4-dihydro-5-methyl-2-phenyl-4-(phenylazo)-3H-pyrazol-3-one
- Details on test material:
- purity: 98.9 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Stock solution: The solution (100 mg/L test item was weighed out) was prepared with dilution water in brown glass flask one day day prior to
application.
Dispersion treatment: The stock solution was shaken with 20 rpm for 24 h (rotating shaker 3040, GFL). Undissolved particles were removed by
membrane filtration (0.45 μm, RC, SCHLEICHER & SCHUELL).
Limit concentration: Saturated solution
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene
- Breeder: DR.U.NOACK-LABORATORIEN, 31157 Sarstedt, Germany
- Culture: In 2-3 L glass vessels with approximately 1.8 L culture medium, at 20 ± 2 °C, in an incubator, 16 h illumination, illumination
strength max. 20 μE⋅m-2 ⋅s-1
- Culture medium: Elendt M4, according to Elendt (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Feeding during test: no
- Age of the test organism: 2 - 24 h
ACCLIMATION
- Acclimatisation: at least 2h in dilution water
- Feeding: At least 5 per week ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density
of > 106 cells/mL.
Number of test organisms per concentration and control:
20 animals, divided into 4 parallel samples, each with 5 animals
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 263 mg CaCO3/L
- Test temperature:
- 18 - 22 °C
- Dissolved oxygen:
- 8.42 mg/L
- Nominal and measured concentrations:
- nominal 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Type (delete if not applicable): open
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: According to Directive 92/69/EC L383A C.2. Annex
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μE⋅m-2 ⋅ s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Preliminary study:
with 1:1, 1:10. 1.100 dilutions with 10 daphnids:
no immobilisation after 24 h and 48 h - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immoblisation
- Remarks on result:
- other: saturated solution
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 h. The EC50- with
95 % confidence interval (CI) was determined by sigmoidal dose-response regression. ECvalues
after 24 h of the reference item in mg/L:
EC50 : 1.95 (CI 1.71 - 2.16)
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed
concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for
daphnids clone 5 cultured in Elendt M4 medium.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution of the test item no biologically significant effect to Daphnia Magna effect was determined
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution# (DOC 3.4 mg/L) of the test item was determined according to OECD 202 (2004) . The limit test was conducted under static conditions over 48 h . 20 test organisms were exposed to the saturated solution and the control.
A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 1.95 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
The test item was visually clear dissolved at the tested saturated solution throughout the exposure period. No immobility was determined in the tested saturated solution after 24 h and 48 h. There is no biologically significant effect neither in the tested saturated solution nor in the control group.
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