Registration Dossier
Registration Dossier
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EC number: 233-139-2 | CAS number: 10043-35-3
Sediment toxicity
Administrative data
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well performed and documented study according to GLP and international guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: whole sediment chronic toxicity testing of boron with the freshwater fatmucket mussel.
- Deviations:
- no
- Remarks:
- based on ASTM E2455-06 (ASTM, 2006), OECD 25 (OECD, 2007), Simon (2005), with use of natural sediment in place of reconstituted sediment
- Principles of method if other than guideline:
- test methods followed those outlined in the previously approved study protocol
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Boric acid
- EC Number:
- 233-139-2
- EC Name:
- Boric acid
- Cas Number:
- 10043-35-3
- Molecular formula:
- H3BO3
- IUPAC Name:
- boric acid
- Test material form:
- not specified
- Details on test material:
- - Purity: >= 99,5 %
- Source: Sigma Aldrich chemical company
- Lot: 070M0003V
Constituent 1
Test organisms
- Test organisms (species):
- other: Lampsilis siliquoidea
- Details on test organisms:
- - Source: Missouri State University
- Gravid femal L. siliquoidea were collected in Missouri and glochidia obtained were inoculated onto largemouth bass at the MSU propagation facility
- Juvenile L.siliquoidea were reared at MSU in semi-closed systems for approx. 2 months at temperatues maintained between 20 and 25 °C in St Joseph river water.
- Feeding was based on recommendations of the ASTM (2006) guidelines and consisted of a suspension mixture of Nannochloropsis algae and reed mariculture shelfish diet.
Study design
- Study type:
- laboratory study
- Test type:
- semi-static
- Water media type:
- freshwater
- Type of sediment:
- natural sediment
Exposure duration
- Duration:
- 21 d
- Exposure phase:
- total exposure duration
Test conditions
- Hardness:
- 74,4 - 111,2 mg CaCO3/L
- Test temperature:
- 19,1 - 20,6 °C
- pH:
- 6,21 - 7,60
- Dissolved oxygen:
- 5,9 - 9,2 mg/L
- Ammonia:
- 0,02 - 0,46 mg/L as N
- Nominal and measured concentrations:
- 0 (control), 81.6, 143.5, 254.8, 452.4, 806.5 mg B/kg sediment dw
sediment concentrations were maintained by spiing boric acid to the overlying water - Details on test conditions:
- TEST SYSTEM
- Control water: recon moderately hard lab water (US EPA, 2002)
- Overlying waters were spiked with boric acid to minimize dissolution and removal of boron from the spiked sediments
- Overlying waters were renewed daily at 20% of the total overlying water volume to ensure adequate water quality
EXPOSURE REGIME
- 10 L.siliquoidea per replicate
- 4 individual replicates
TEST CHARACTERISTCS
- Alkalinity: 20 to 88 mg CaCO3/L
- Photo period: 16hf light / 8hr dark
- Light intensity: 238-490 lux
CHARACTERIZATION OF SEDIMENT
- Lot WBS102910
- no sieving was conducted
- 7,7% clay, 76,9 % silt and 15,4 % sand
- TOC 11,5 %
- Background boron concentration: 7,9 mg B/kg dry sediment
- 12,4 % dry weight
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 284 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- mortality
- Remarks:
- (survival)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 80.6 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- other: biomass (shell length)
- Details on results:
- NOEC values are below the IC25 value due to high test precision
- Reported statistics and error estimates:
- Shapiro-Wilk's and Bartlett's test: normal distribution (p>0,01)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All test acceptability criteria specified in the study protocal were met.
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