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EC number: 260-906-9 | CAS number: 57693-14-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- Trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- EC Number:
- 260-906-9
- EC Name:
- Trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- Cas Number:
- 57693-14-8
- Molecular formula:
- C40H20CrN6O14S2.3Na
- IUPAC Name:
- trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- Test material form:
- solid: particulate/powder
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9 mix
- Test concentrations with justification for top dose:
- 0-8-40-20-1000-5000 µg/plate + 5 different positive control
Repeat: 0-125-250-500-1000-2000-4000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- other: nitro furatoin (TA 100); 4-nitro-1,2-phenylenediamine(TA 1537, TA98); 2- aminoanthracene
- Details on test system and experimental conditions:
- DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 4
DETERMINATION OF CYTOTOXICITY
- Method: background lawn density - Evaluation criteria:
- Reproducible and dose-related increase of mutant count in at least one strain is considered a positive result.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2
- Remarks:
- not tested according to the OECD guideline 471 in force at the time the test was run.
- Metabolic activation:
- with and without
- Genotoxicity:
- not determined
- Cytotoxicity / choice of top concentrations:
- not determined
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Summary of mean values without S9 mix
dose (µg/plate) |
TA1535 |
TA100 |
TA1537 |
TA 98 |
0 8 40 200 1000 5000 Na N3 NF 4 -NPDA |
15 15 13 14 13 P 537 |
110 117 118 112 125 P 243 |
12 11 14 17 32 P 45 |
28 34 38 50 68 P 65 |
0 125 250 500 1000 2000 4000 Na N3 NF 4 -NPDA |
12 10 10 14 7 11 P 747 |
102 92 98 108 115 109 P 460
|
9 19 17 19 30 23 P 74 |
32 37 37 38 46 43 P 129 |
0 400 600 800 1000 1200 1400 1600 4 -NPDA |
|
|
6 22 20 24 34 32 31 21 83 |
33 52 56 56 66 65 59 62 291 |
Summary of mean values with S9-mix
dose (µg/plate) | TA 1535 | TA100 | TA 1537 | TA 98 |
30 % S9mix 0 8 40 200 1000 5000 2 -AA |
22 29 25 23 18 P 152 |
159 165 182 172 192 P 663 |
15 11 14 15 23 P 75 |
34 46 38 57 85 P 464 |
10 % S9mix 0 125 250 500 1000 2000 4000 2 -AA |
15 17 14 15 15 11 P 253 |
126 145 153 124 109 107 P 934 |
13 14 13 18 23 22 P 443 |
36 54 55 53 50 42 P 1304 |
30 % S9mix 0 400 600 800 1000 1200 1400 1600 2 -AA |
13 12 14 15 26 22 18 24 116 |
52 71 83 83 87 83 82 65 586 |
Applicant's summary and conclusion
- Conclusions:
- Test substance showed equivocal mutagenic effects in 2 of 4 strains in Salmonella typhimurim.
- Executive summary:
Method
The test material was examined in four strains of Salmonella typhimurium for gene mutations in bacteria (Ames test) in the presence and absence of a metabolic activation system (rat liver S9 mix). The strains employed were TA 98, TA 100, TA 1535, and TA 1537. All concentrations were tested in four plates each. The dose range was 0 (vehicle), 8 to 5000 µg/plate and several positive controls.
Results
Positive as well as negative controls gave the expected results. Plates treated with test item showed precipitation at concentrations of 4000 µg/plate and above. Cytotoxicity was noted at doses above 800 µg/plate. Equivocal mutagenic effects were observed in TA 1537 and TA 98. There were weak increases of revertant colonies, less than three times, mostly less than twice, the negative controls. However, no clear dose response relations could be determined, even when the dose was increased significantly.
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