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EC number: 240-347-7 | CAS number: 16219-75-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- Comparable to guideline study. Original study report not available.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Method of Ames (1975)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 5-ethylidene-8,9,10-trinorborn-2-ene
- EC Number:
- 240-347-7
- EC Name:
- 5-ethylidene-8,9,10-trinorborn-2-ene
- Cas Number:
- 16219-75-3
- Molecular formula:
- C9H12
- IUPAC Name:
- 5-ethylidenebicyclo[2.2.1]hept-2-ene
- Details on test material:
- - Test substance: 5-Ethylidene-2-norbornene (CAS# 16219-75-3)
- Purity: 99.4%
- Source: Union Carbide Corporation, South Charleston, West Virginia,
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver homogenate, prepared from Aroclor l254-induced, Sprague-Dawley male rats, was purchased from Microbiological Associates, Bethesda, MD. For tests with metabolic activation, 0.5 ml of S9 mix containing 50 microL of S9 was added per plate.
- Test concentrations with justification for top dose:
- 0.001-0.1 mg/plate
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 4-nitro-o-phenylenediamine, 2-aminoanthracene (depending on strain)
- Details on test system and experimental conditions:
- ENB was tested in triplicate at each of 5 doses. Either 0.5 mL sodium phophate buffer or S9/bacteria mix was added to the tubes containing 2 mL top agar, 100 uL bacterial strain and 100 uL of ENB, control substance, or solvent. The contents of the tubes were poured on agar plates which were allowed to harden. Plates were then incubated for 48-72 hours at 37C in a darkened incubator. Revertant colonies were counted by automatic colony counter.
- Evaluation criteria:
- The test substance was considered positive if it produced at least a two-fold increase in the number of revertant colonies compared to the negative control, and there is evidence for a dose-related increase in the number of revertant colonies.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: yes in all strains except TA1535 without metabolic activation. Only in TA100 with metabolic activation.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No indication of mutagenicity was observed at any of the tested doses, either by evidence of a dose-response relationship or a doubling of the number of colonies over the solvent control.
Any other information on results incl. tables
A preliminary toxicity/initial mutagenicity assay was conducted with 10 doses over a range of 0.01 to 90 mg/mL using strain TA100 with and without S-9. Toxicity was exhibited at dosages in the range of 1-99 mg/plate. Dosages of 0.1 and 0.3 mg/plate allowed only sparse growth. The dosages used in the definitive study were chosen to span the range 0.001-0.1 mg/plate. None of the five strains, with or without induced rat liver S-9, exhibited reversion frequencies substantially different from spontaneous controls in this assay.
In the test without metabolic activation, toxicity was observed at 0.l mg/plate with all strains except TAl535. In the test with metabolic activation, toxicity was observed at 0.1 mg/plate in both strains TA100 and TA1538. All strains exhibited a positive mutagenic response with the positive controls tested both with and without S9 metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative. The test substance was not mutagenic with or without metabolic activation in this test system.
- Executive summary:
In an Ames test ENB did not produce a mutagenic response in the Salmonella/microsome mutagenicity assay when tested with or without metabolic activation.
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