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EC number: 200-289-5 | CAS number: 56-81-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted prior to GLP and test guidelines, but sufficient data is available for interpretation of results
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparative toxicity of synthetic and natural glycerin
- Author:
- Hine, C
- Year:
- 1 953
- Bibliographic source:
- Arch Ind Hyg Occup Med 7:282-291
Materials and methods
- Principles of method if other than guideline:
- Groups of rats were dosed orally and observed for 10 days. Gross necropsy was performed on all animals.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Glycerol
- EC Number:
- 200-289-5
- EC Name:
- Glycerol
- Cas Number:
- 56-81-5
- Molecular formula:
- C3H8O3
- IUPAC Name:
- propane-1,2,3-triol
- Details on test material:
- CAS 56-81-5 (glycerine)
Natural glyerine, achieved from market stock, although purity was not indicated (impurities were fatty acids and esters) it was USP grade material.
Synthetic glycerin, purity 99.5% (rest mainly water with very small amounts of glycerin polymers and glyceraldehyde)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Long-Evans
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Rats were obtained from local suppliers and were quarantined for about two weeks prior to use. Metal cages bedded with sawdust housed them in approximately equal groups, which were separated according to species and sex. The temperature of the animal room varied between 15 and 17C. The animals were fed commercial animal food pellets of known composition, supplemented weekly with greens.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- TEST ORGANISMS:
- Number: 12
- Mean weight at study initiation: 114 g
ADMINISTRATION:
- Dose: 27.26 g/kg bw
- Route: oral (gavage) after fasting 22 to 24 hours. Feed was offered two hours after the treatment and water was available at all times.
- Vehicle: none (undiluted)
- Post dose observation period: 10 days
- Doses:
- 27260 mg/kg (only dose tested; reported by von Oettingen to be the LD50.
Reference
van Oettingen, W.F. (1943). The aliphatic alcohols: Their toxicity and potential dangers in relation to their chemical constitution and their fate in metabolism. Public Health Bulletin 281, U.S. Public Health Service. - No. of animals per sex per dose:
- 12 females/dose level
- Control animals:
- not specified
- Details on study design:
- EXAMINATIONS: mortality, clinical signs, body weight (frequency not indicated), macroscopy in animals that died and selected survivors, histopathology of brain, heart, liver, spleen, stomach, intestine and kidney.
- Statistics:
- STATISTICAL METHOD: LD50 was calculated using logarithmic-probit graph paper
Results and discussion
- Preliminary study:
- Not applicable.
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 27 200 mg/kg bw
- Based on:
- test mat.
- Mortality:
- MORTALITY: not indicated
NECROPSY FINDINGS: - Clinical signs:
- other: Muscle spasms and clonic convulsions prior to death. Survivors appeared normal within 2.5 hours after administration.
- Gross pathology:
- Hyperaemia of pylores and small intestine; congestion of the lungs; pale spleen; 3 animals showed hyperaemia of the cerebral meninges
- Other findings:
- No additional information provided.
Any other information on results incl. tables
No additional information available.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Results for natural and synthetic glycerine were comparable with an oral LD50 of 27,200 mg/kg.
- Executive summary:
The acute oral LD50 of glycerine was examined. Results for natural and synthetic glycerine were comparable with an oral LD50 of 27,200 mg/kg.
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