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EC number: 233-032-0 | CAS number: 10024-97-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- fertility, other
- Remarks:
- based on test type (migrated information)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, no TG followed. One-generation exposure
Data source
Reference
- Reference Type:
- publication
- Title:
- The effects of intermittent chronic exposure of nitrous oxide on rat fertility and pregnancy
- Author:
- Webman, S.
- Year:
- 1 980
- Bibliographic source:
- Pediatric Dentistry, 2(3); p 208-216
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The effects of intermittent chronic exposure of nitrous oxide on rat fertility and pregnancy over one-generation exposure
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Dinitrogen oxide
- EC Number:
- 233-032-0
- EC Name:
- Dinitrogen oxide
- Cas Number:
- 10024-97-2
- Molecular formula:
- N2O
- IUPAC Name:
- Dinitrogen Oxide
- Test material form:
- gas under pressure: liquefied gas
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure (if applicable):
- whole body
- Vehicle:
- air
- Details on mating procedure:
- After 30 d of exposure rats were mated as follows: a) 7 pairs from N2O exposed M and F; b) 6 pairs from N2O exposed M and O2 exposed females, c) 6 pairs from O2 exposed M and N2O exposed F, d) 6 pairs from O2 exposed M and F, e) 5 pairs from unexposed M & F.
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 30 d prior to mating and then over one generation
- Frequency of treatment:
- 2h/d
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 70%
Basis:
nominal conc.
- No. of animals per sex per dose:
- 12/gp
- Control animals:
- yes, concurrent no treatment
Results and discussion
Results: P0 (first parental generation)
Effect levels (P0)
- Dose descriptor:
- NOAEC
- Effect level:
- > 700 000 ppm
- Sex:
- male/female
- Basis for effect level:
- other: No effects on fertility
Results: F1 generation
Effect levels (F1)
- Dose descriptor:
- NOAEC
- Generation:
- F1
- Effect level:
- > 700 000 ppm
- Sex:
- male/female
- Basis for effect level:
- other: No effect on litter size or pups
Overall reproductive toxicity
- Reproductive effects observed:
- not specified
Any other information on results incl. tables
A total of 22 litters were born to 30 dams, distributed almost evenly amongst the pairings. No gross developmental deformities were noted in either the treated or control group animals. Chi-square analysis shows no significant difference in the no. of litters produced by exposed and unexposed pairings.
Histological examination of the ovaries and testes showed no demonstrable difference between experimental and control animals, no focal or generalised destruction was observed in the testes or ovaries of treated animals even when exposure to N2O was for 30 d.
Dams were allowed to deliver pups. Pups were weighed, measured and examined for abnormalities. No effects on fertility, litter size or pups were found in this study.
Applicant's summary and conclusion
- Conclusions:
- Dams were allowed to deliver pups. Pups were weighed, measured and examined for abnormalities. No effects on fertility, litter size or pups were found in this study.
As animals were exposed to a single dose concentration of N2O, the objective therefore of the study was not to establish a NOAEC. It however could be concluded that the NOAEC was >70% as no effects on fertility were observed. - Executive summary:
Groups of 22 rats were exposed via whole body inhalation to 70% N2O / 30% O2 for 2 h/for 30 consecutive days. A group of 12 rats of each sex exposed to O2 only over the same schedule. There was a further unexposed control group of 5 rats/sex. Two rats of each sex exposed to N2O were killed at 5, 01, 20 or 30 d of treatment and the gonads removed and fixed in formalin. One rat of each sex from the same group was killed before the start of exposure to provide a histological baseline. After 30 d of exposure rats were mated as follows: a) 7 pairs from N2O exposed M and F; b) 6 pairs from N2O exposed M and O2 exposed females, c) 6 pairs from O2 exposed M and N2O exposed F, d) 6 pairs from O2 exposed M and F, e) 5 pairs from unexposed M & F.
Dams were allowed to give birth to their litters and pups were weighed measured for abnormalities.
A total of 22 litters were born to 30 dams, distributed almost evenly amongst the pairings. No gross developmental deformities were noted in either the treated or control group animals. Chi-square analysis shows no significant difference in the no. of litters produced by exposed and unexposed pairings.
Histological examination of the ovaries and testes showed no demonstrable difference between experimental and control animals, no focal or generalised destruction was observed in the testes or ovaries of treated animals even when exposure to N2O was for 30 d.
Dams were allowed to deliver pups. Pups were weighed, measured and examined for abnormalities. No effects on fertility, litter size or pups were found in this study.
As animals were exposed to a single dose concentration of N2O, the objective therefore of the study was not to establish a NOAEC. It however could be concluded that the NOAEC was >70% as no effects on fertility were observed.
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