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EC number: 221-220-5 | CAS number: 3033-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1975
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Range-finding study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N,N,N',N'-tetramethyl-2,2'-oxybis(ethylamine)
- EC Number:
- 221-220-5
- EC Name:
- N,N,N',N'-tetramethyl-2,2'-oxybis(ethylamine)
- Cas Number:
- 3033-62-3
- Molecular formula:
- C8H20N2O
- IUPAC Name:
- {2-[2-(dimethylamino)ethoxy]ethyl}dimethylamine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- CAS 3033-62-3 (2,2'oxybis(N,N-dimethyl ethanamine),
purity not indicated
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Harlan Wistar Albino
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- - Age: 30 days of age at study initiation
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- other: dietary feed
- Details on oral exposure:
- -The test substance was added to ground Purina Laboratory Chow and fed in the diet
- Post exposure period: none - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- - Dosage goal 0, 0.15, 0.35, and 0.85 gm/kg
- Concentration in diet:
males: 0, 0.13, 0.28, 0.69%;
females:0.14, 0.33, 0.80%
- Diet consumed:
males: 17.8, 10.1, and 5.4 gm/rat/day;
females: 14.8, 8, 4.4 gm/rat/day
- Dosage attained:
males: 0.15, 0.22, and 0.33 gm/kg/day;
females: 0.15, 0.22, and 0.32 gm/kg/day - Duration of treatment / exposure:
- 7 days
- Frequency of treatment:
- Daily
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Dose / conc.:
- 150 mg/kg bw/day (nominal)
- Dose / conc.:
- 220 mg/kg bw/day (nominal)
- Dose / conc.:
- 320 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- yes
- Positive control:
- none
Examinations
- Observations and examinations performed and frequency:
- - Clinical signs and mortality: daily
- Body weight: on day 1 (prior to exposure), 5, and 7 (prior to termination)
- Food consumption: monitored daily - Sacrifice and pathology:
- ORGANS EXAMINED AT NECROPSY (GROSS AND MICROSCOPIC):
- Organ weights: liver, kidneys
- Gross pathology: Lungs, Liver, Kidneys, and Spleen
- Microscopic examination: lung, liver, kidneys, heart, spleen, adrenal, thyroids, parathyroids, trachea, esophagus, stomach, duodenum, pancreas, colon, urinary bladder, brain, pituitary and prostate, testicle, epididymis, uterus and ovary from high dosage and control groups; lung, liver, kidneys, heart, spleen, adrenal, thyroids, parathyroids, trachea, esophagus and brain from the two lower dosage groups - Statistics:
- Not specified
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- 1 female and 2 males from the high dose group; all other animals survived
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- significantly depressed body weight in both males and females at 220 and 320 mg/kg/day
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Description (incidence and severity):
- concentration-related reduction in both sexes
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Description (incidence and severity):
- Significant decrease in kidney weight in males at 220 and 320 mg/kg/day; slight increase in liver weight in males at 320 mg/kg/day; significant increase in liver weight relative to body weight in males and females at 320 mg/kg/day; decreased absolute kidney weights in females at 320 mg/kg/day; At the 220 mg/kg/day level, liver weights of males were depressed (opposite of effect at 320 mg/kg/day)
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Treatment-related findings were limited to the skin and kidneys
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Lesions of the lung, liver and stomach were associated with the two highest dosage levels; interstitial proliferative pneumonia, central fat vacuolization of hepatic cord cells and vacuolated smooth muscle fibers, respectively. No significant effects were associated with 150 mg/kg/day.
- Histopathological findings: neoplastic:
- no effects observed
- Other effects:
- not specified
Effect levels
- Dose descriptor:
- NOEL
- Remarks:
- systemic toxicity
- Effect level:
- 150 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- histopathology: non-neoplastic
- organ weights and organ / body weight ratios
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- In this K4, dose range finding study in rats, an NOEL for systemic toxicity is established at 150 mg/kg/day . An LOAEL for local effects is established at 220 mg/kg/day.
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