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EC number: 212-298-1 | CAS number: 778-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
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- Irritation / corrosion
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Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Mar – 21 Jun 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- GLP compliance:
- yes
- Remarks:
- The test was performed in compliance with the requirements of the Principles of Good Laboratory Practices (GB/T 22278-2008).
Test material
- Reference substance name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- EC Number:
- 212-298-1
- EC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- Cas Number:
- 778-94-9
- Molecular formula:
- C8H3F3N2O2
- IUPAC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: During the test, certain quantities of test substance were weighed directly and added to the test bottles.
- Controls: Yes. Reference compound 3,5-dichlorophenol stock solution (1000 mg/L): 0.1002 g 3,5-dichlorophenol was weighed, diluted with deionised water to approximately 100 mL, yielding 3,5-dichlorophenol solution with a pH adjusted to 7.82 and a concentration of 1000 mg/L (Positive control). Inoculum blank control without test substance.
- Test substance concentrations: 0.0 (blank control), 10, 20, 40, 80, and 160 mg/L
- Reference compound concentrations: 3, 10, and 30 mg/L
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Nanjing Tiebei Sewage Treatment Plant
- Method of cultivation: The sewage sludge was washed with deionised water, centrifuged at high-speed for 10 minutes at 5000 r/min, and the supernatant poured out. The operation was repeated three times, a small quantity of washed sludge was weighed and dried, and dry sludge content calculated to be 6.79%. Based on sludge dry-weight content, 265.30 g of centrifuged wet sludge (sludge dry weight 18.0 g) was added to 300 mL synthetic sewage, and deionised water used to dilute to 6 L, yielding a 3.00 g/L activated sludge suspension, and the wet sludge stirred for 2 minutes to homogenise.
- Preparation of inoculum for exposure: Before the test started, the activated sludge suspension was aerated for 16 h at (20 ± 2) °C; the measured pH value was 7.41.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.62 - 7.96
- Dissolved oxygen:
- 0.07 - 7.97
- Details on test conditions:
- TEST SYSTEM
- Aeration: Aerated with continuous stirring. Clean, de-oiled air.
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- The respiration rate of the test solution was measured every 15 minutes
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.0 (blank control), 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study: Dissolved oxygen before and after the abiotic control test was 6.71 mg/L and 6.71 mg/L respectively; there were no pronounced abiotic aerobic effects. Test substance 3 h-EC50 = 103 mg/L, the 95% confidence interval was 66.9 mg/L - 159 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Total respiration rate for the blank control was 46.1 mgL-1 h-1 - 48.1 mgL-1 h-1, which converts to an activated sludge (1.5 g/L) respiration rate of 30.8 mgg-1 h-1 - 32.0 mgg-1 h-1 (> 20 mgg-1 h-1), and the coefficient of variation was 1.49% (< 30%).
The respiration rates for inoculum blank controls without ATU and with ATU were 41.1 mgL-1 h-1 and 39.6 mgL-1 h-1; inhibition rate for nitrification respiration was 3.57% (< 5%), indicating that nitrification effects were not significant, and that this could be used in the actual test. - Results with reference substance (positive control):
- The 3 h total respiration inhibition EC50 value for the reference compound 3,5-dichlorophenol was 13.1 mg/L, the 95% confidence interval was 11.4 mg/L - 15.0 mg/L (between 2-25 mg/L).
Any other information on results incl. tables
Table 1: Total Respiration Inhibition Test Results
Treatment |
Conc. (mg/L) |
Dissolved oxygen (mg/L) |
Time (s) |
Respiration rate (mgL-1h-1) |
Inhibition rate (%) |
|
Upper limit |
Lower limit |
|||||
1 Blank control FB1 |
0 |
7.25 |
0.07 |
540 |
47.9 |
— |
2 Test substance FT1-1 |
10.1 |
7.35 |
0.37 |
540 |
46.5 |
1.62 |
3 Test substance FT1-2 |
10.0 |
7.24 |
0.24 |
540 |
46.7 |
1.34 |
4 Test substance FT1-3 |
10.1 |
7.53 |
0.63 |
540 |
46.0 |
2.75 |
5 Test substance FT1-4 |
10.1 |
7.14 |
0.27 |
540 |
45.8 |
3.17 |
6 Test substance FT1-5 |
10.3 |
7.26 |
0.33 |
540 |
46.2 |
2.33 |
7 Test substance FT2-1 |
20.1 |
7.97 |
1.57 |
540 |
42.7 |
9.80 |
8 Test substance FT2-2 |
19.9 |
7.55 |
1.66 |
540 |
39.3 |
17.0 |
9 Test substance FT2-3 |
19.9 |
7.31 |
1.15 |
540 |
41.1 |
13.2 |
10 Test substance FT2-4 |
19.9 |
7.23 |
1.03 |
540 |
41.3 |
12.6 |
11 Test substance FT2-5 |
20.1 |
7.37 |
1.06 |
540 |
42.1 |
11.1 |
12 Test substance FT3-1 |
40.0 |
7.18 |
5.68 |
540 |
10.0 |
78.9 |
13 Test substance FT3-2 |
40.2 |
7.86 |
6.69 |
540 |
7.80 |
83.5 |
14 Test substance FT3-3 |
40.2 |
7.51 |
6.30 |
540 |
8.07 |
82.9 |
15 Test substance FT3-4 |
39.8 |
7.46 |
6.03 |
540 |
9.53 |
79.8 |
16 Test substance FT3-5 |
40.0 |
7.38 |
5.97 |
540 |
9.40 |
80.1 |
17 Reference control FR4 |
3.00 |
7.37 |
0.55 |
540 |
45.5 |
3.88 |
18 Reference control FR5 |
10.0 |
7.28 |
2.79 |
540 |
29.9 |
36.7 |
19 Reference control FR6 |
30.0 |
7.57 |
6.43 |
600 |
6.84 |
85.5 |
20 Blank control FB2 |
0 |
7.12 |
0.09 |
540 |
46.9 |
— |
21 Blank control FB3 |
0 |
7.56 |
0.48 |
540 |
47.2 |
— |
22 Blank control FB4 |
0 |
7.88 |
0.77 |
540 |
47.4 |
— |
23 Test substance FT4-1 |
80.0 |
7.20 |
6.61 |
600 |
3.54 |
92.5 |
24 Test substance FT4-2 |
80.0 |
7.27 |
6.12 |
600 |
6.90 |
85.4 |
25 Test substance FT4-3 |
80.2 |
7.21 |
6.60 |
600 |
3.66 |
92.3 |
26 Test substance FT4-4 |
80.0 |
7.28 |
6.64 |
600 |
3.84 |
91.9 |
27 Test substance FT4-5 |
80.2 |
7.22 |
6.55 |
600 |
4.02 |
91.5 |
28 Test substance FT5-1 |
160 |
7.28 |
7.00 |
600 |
1.68 |
96.4 |
29 Test substance FT5-2 |
160 |
7.89 |
7.41 |
600 |
2.88 |
93.9 |
30 Test substance FT5-3 |
160 |
7.55 |
7.32 |
600 |
1.38 |
97.1 |
31 Test substance FT5-4 |
160 |
7.19 |
6.98 |
600 |
1.26 |
97.3 |
32 Test substance FT5-5 |
160 |
7.33 |
7.14 |
600 |
1.14 |
97.6 |
33 Reference control FR4 |
3.0 |
7.66 |
0.70 |
540 |
46.4 |
1.90 |
34 Reference control FR5 |
10 |
7.38 |
2.75 |
540 |
30.9 |
34.7 |
35 Reference control FR6 |
30 |
7.41 |
6.46 |
600 |
5.70 |
87.9 |
36 Blank control FB5 |
0 |
7.64 |
0.72 |
540 |
46.1 |
— |
37 Blank control FB6 |
0 |
7.82 |
0.61 |
540 |
48.1 |
— |
Test substance EC50 |
35.6 (mg/L) |
Test substance EC50 95% confidence interval (mg/L) |
31.9 – 39.7 mg/L |
|||
Reference compound EC50 |
13.1 (mg/L) |
Reference compound EC50 95% confidence interval (mg/L) |
11.4 – 15.0 mg/L |
Notes: Coefficient of variation for the respiration rate of the inoculum blank control was 1.49% (within 30%), total respiration rate of the blank control was 46.1mgg-1h-1- 48.1mgL-1h-1, which converts to an activated sludge (1.5 g/L) respiration rate of 30.8mgg-1h-1- 32.0 mgg-1h-1.
Table 2: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
Total respiration rate for the blank control was 30.8 mg*g-1*h-1- 32.0 mg*g-1*h-1. |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
The coefficient of variation of oxygen uptake rate in control replicates was 1.49% (< 30%). |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 2 in "Any other information on results incl. tables".
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