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EC number: 201-939-0 | CAS number: 89-78-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-03-14 till 2000-05-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch 29090020
OTHER SPECIFICS: Name of the test substance: Menthol rac. - Analytical monitoring:
- yes
- Details on test solutions:
- The test substance was weighed into water and treated for 1 h with an ultrasonic batch to produce the stock stock solution (50 mg/L)
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Origin: Institute of Plant Physiology, University of Goettingen
- Cultivation: light chamber at 23 +- 2 °C and 120 µE/m2s
- Algal inoculum 10000 cells/ml initial cell density
- Illumination: 120 µE/m2s
- Cell density measured with a microcellcounter after 24, 48 and 72 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 +- 2 °C
- pH:
- 7.5 - 10.3
- Nominal and measured concentrations:
- Nominal concentration: 1.25, 2.5, 5, 10, 20 and 40 mg/L (Test concentrations 1.25 and 2.5 mg/L were not analytically determined as the expected concentrations were below the detection limit of the TOC determination method (2 mg/L).
Not measured: 1.25 and 2.5 mg/L
Measured (0 h): 4, 8, 15, 29 mg/L
Measured (72 h) 3, 7, 13, 26 mg/L - Details on test conditions:
- - Erlenmeyer flasks (300 mL) with stoppers
- 100 mL of test medium
- Light chamber with shaker - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 15.504 - 16.537 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Since the original study on short-term toxicity to algae from 2000 only gives a range (> 9.8 < 18.2 mg/L) but not a distinct value for the ErC50 this value was determined by Probit analysis as ErC50 = 16.2 mg/L and quoted in the OECD SIDS report, 2003 (agreed by the ICCA initiative). The calculated ErC50 value is used as result of the underlying study on short-term toxicity to algae.
The NOEC results from the Dunnett test. - Validity criteria fulfilled:
- yes
- Remarks:
- The mean of the replicate coefficients of variation in the section-by-section growth rate was 20 %; - The coefficient of variation of the mean specific rate replicates in the control not exceed 7 %
- Conclusions:
- During 72 hours exposure an ErC50 of 16.2 mg/L and a NOEC of 4.6 mg/L was determined.
- Executive summary:
A growth inhibition study according to EU Method C.3 on algae which is equivalent to OECD Guideline for Testing of Chemicals No. 201 was performed on Desmodesmus subspicatus (former name: Scenedesmus subspicatus). Algae were exposed to 6 concentration levels (1.25, 2.5, 5, 10, 20 and 40 mg/L) of DL-Menthol for 72 h and one control without substance was performed. Cell density and pH were determined every 24 h. A 72-hour EC50 of 16.2 mg/L based on nominal concentrations was determined and NOEC resulting from Dunnett was 4.6 mg/L.
Reference
Results analysis by start of test and after 72 hours
Nominal concentration [mg/L] | 0 hours [mg/L] | 72 hours [mg/L] |
control | < 2 | 3 |
5 *w.algae | 5 | 3 |
5 | 4 | 3 |
10 | 8 | 7 |
20 | 15 | 13 |
40 | 29 | 26 |
40 *w.algae | 27 | 27 |
*w.algae = without algal inoculum
All values refer to TOC determinations. According to the relevant product infomations 1 mg/L TOC equals to 1.3 mg/L of the test substance (empirical formula: C10H20O; molecular weight: 156.3 g/mol).
Analysis of the test concentrations 5 mg/L, 10 mg/L, 20 mg/L, 40 mg/L plus a control; additional analysis of the highest and the lowest analytically determined test concentration without algal inoculum, at test start and after 72 hours of exposure.
The test concentrations 1.25 mg/L and 2.5 mg/L were not analytically determined as the measured concentrations to be expected were below the detection limit of the TOC determination method (2 mg/L).
Before measuring TOC, the samples after 72 hours of exposure were filtered.
Description of key information
A growth inhibition study according to EU Method C.3 on algae which is equivalent to OECD Guideline for Testing of Chemicals No. 201 was performed on Desmodesmus subspicatus (former name: Scenedesmus subspicatus). Algae were exposed to 6 concentration levels (1.25, 2.5, 5, 10, 20 and 40 mg/L) of DL-Menthol for 72 h and one control without substance was performed. Cell density and pH were determined every 24 h. A 72-hour EC50 of 16.2 mg/L based on nominal concentrations was determined and NOEC resulting from Dunnett was 4.6 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 16.2 mg/L
- EC10 or NOEC for freshwater algae:
- 4.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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