Registration Dossier
Registration Dossier
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EC number: 204-269-7 | CAS number: 118-69-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,6-dichlorotoluene
- EC Number:
- 204-269-7
- EC Name:
- 2,6-dichlorotoluene
- Cas Number:
- 118-69-4
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 1,3-dichloro-2-methylbenzene
- Details on test material:
- - Analytical purity: 996%
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 : Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- -S9 mix; 0, 4.69, 9.38, 18.8, 37.5, 75.0, 150 μg/plate (TA100, TA98, TA1537, WP2 uvrA)
-S9 mix; 0, 2.34, 4.69, 9.38, 18.8, 37.5, 75.0, 150 μg/plate (TA1535)
+S9 mix; 0, 18.8, 37.5, 75.0, 150, 300, 600 μg/plate (TA100, TA98, TA1537, WP2 uvrA)
+S9 mix; 0, 9.38, 18.8, 37.5, 75.0, 150, 300, 600 μg/plate (TA1535) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide
- Remarks:
- without S9 mix (TA100, TA98, WP2 uvrA)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9 mix (TA1535)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9 mix (TA1537)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- with S9 mix (five strains)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
2,6-Dichlorotoluene did not induce gene mutations in S. typhimurium TA100, TA98, TA1535, TA1537 and E. coli WP2 uvrA strains with or without S9 mix. - Executive summary:
2,6-Dichlorotoluene did not induce gene mutations in S. typhimurium TA100, TA98, TA1535, TA1537 and E. coli WP2 uvrA strains with or without S9 mix.
Genetic effects:
Salmonella typhimurium TA100, TA1535, TA98, TA1537
+ ? - Without metabolic activation: [ ] [ ] [*] With metabolic activation: [ ] [ ] [*] Genetic effects:
Escherichia coli WP2 uvrA
+ ? - Without metabolic activation: [ ] [ ] [*] With metabolic activation: [ ] [ ] [*]
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