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EC number: 207-980-0 | CAS number: 504-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 1 Feb 2008 and 29 Feb 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP and in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do no effect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of inspection: 21-08-2007 Date of Signature: 15-10-2007
Test material
- Reference substance name:
- Cyclohexane-1,3-dione
- EC Number:
- 207-980-0
- EC Name:
- Cyclohexane-1,3-dione
- Cas Number:
- 504-02-9
- Molecular formula:
- C6H8O2
- IUPAC Name:
- cyclohexane-1,3-dione
- Details on test material:
- - Name of test material (as cited in study report): 1,3 cyclohexane dione
- Substance type: Powder
- Physical state: Solid
- Analytical purity: 1,3 Cyclohexane Dione >97% W/W
- Impurities (identity and concentrations): Sodium Chloride 1%W/W Maximum
Water 0.5%W/W Maximum
- Composition of test material, percentage of components: 1,3 Cyclohexane Dione 99.2% W/W
Sodium Chloride 0.25%W/W
Water 0.4%W/W
- Purity test date: 18 November 2009
- Lot/batch No.: 184731091224
- Expiration date of the lot/batch: 18 May 2010
- Radiochemical purity (if radiolabelling): N/A
- Stability under test conditions: Stable
- Storage condition of test material: room temperature in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- A mixed population of sewage sludge microorganisms was obtained on 1st February 2008 from the final effluent stage of the Severn Trent Water plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage. The sample of effluent was filtered through coarse filter paper (first approximate 200ml discarded) prior to use.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 1.5 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- N/A
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 28
- Sampling time:
- 28 d
- Remarks on result:
- other: Samples taken at 0, 3, 5, 7, 11, 14, 18, 21, 24 and 28 days
- Details on results:
- The test material attained 28% degradation after 28 days
Any other information on results incl. tables
Culture Medium with Inoculum
Sodium Benzoate (3.0 mg/l) with Inoculum
Test Material (1.5 mg/l) with Inoculum
Test Material (1.5 mg/l) plus Sodium Benzoate (1.5mg/l) with Inoculum
Culture Medium with Inoculum
Sodium Benzoate (3.0 mg/l) with Inoculum
Test Material (1.5 mg/l) with Inoculum
Test Material (1.5 mg/l) plus Sodium Benzoate (1.5 mg/l) with Inoculum
Dissolved oxygen measurements for the test material, standard material and toxicity control preparations together with the controls on Days 0, 3, 5, 7, 11, 14, 18, 21, 24 and 28 are given in Table 1.
Table 1 Dissolved Oxygen Measurements
Test Series |
Dissolved Oxygen (mg O2/l) |
||||||||||
Day |
|||||||||||
0 |
3 |
5 |
7 |
11 |
14 |
18 |
21 |
24 |
28 |
||
R1 |
8.55 |
8.40 |
8.35 |
8.30 |
8.10 |
8.05 |
8.00 |
7.95 |
7.80 |
7.90 |
|
R2 |
8.55 |
8.40 |
8.40 |
8.20 |
8.10 |
7.95 |
7.95 |
8.00 |
7.90 |
7.80 |
|
R1 |
8.50 |
5.70 |
5.45 |
5.30 |
5.30 |
4.90 |
4.60 |
4.60 |
4.70 |
4.70 |
|
R2 |
8.40 |
5.70 |
5.20 |
5.10 |
5.00 |
4.50 |
4.90 |
4.70 |
4.50 |
4.40 |
|
R1 |
8.45 |
8.25 |
8.05 |
7.80 |
7.70 |
7.00 |
7.05 |
7.05 |
7.00 |
6.90 |
|
R2 |
8.40 |
8.30 |
7.95 |
8.05 |
7.80 |
7.20 |
7.10 |
7.05 |
6.90 |
6.90 |
|
R1 |
8.40 |
6.95 |
6.75 |
6.70 |
6.30 |
5.60 |
5.60 |
5.60 |
5.25 |
5.30 |
|
R2 |
8.40 |
7.00 |
6.80 |
6.70 |
6.40 |
5.95 |
5.80 |
5.60 |
5.50 |
5.40 |
R1and R2= Replicates 1 and 2
Oxygen depletion values and the mean percentage degradation values (% of ThOD NO3for the test material and the standard material) are given in Table 2.
Table 2Oxygen Depletion and Mean Percentage Biodegradation Values
Test Series |
Day |
||||||||||
3 |
5 |
7 |
11 |
14 |
18 |
21 |
24 |
28 |
|||
Mean O2Depletion (mg O2/l) |
0.150 |
0.175 |
0.300 |
0.450 |
0.550 |
0.575 |
0.575 |
0.700 |
0.700 |
||
O2Depletion (mg O2/l) |
R1 |
2.650 |
2.875 |
2.900 |
2.750 |
3.050 |
3.325 |
3.325 |
3.100 |
3.100 |
|
R2 |
2.550 |
3.025 |
3.000 |
2.950 |
3.350 |
2.925 |
3.125 |
3.200 |
3.300 |
||
% Degradation (mean) |
52 |
59 |
59 |
57 |
64 |
62 |
64 |
63 |
64 |
||
O2Depletion (mg O2/l) |
R1 |
0.050 |
0.225 |
0.350 |
0.300 |
0.900 |
0.825 |
0.825 |
0.750 |
0.850 |
|
R2 |
0.050 |
0.275 |
0.050 |
0.150 |
0.650 |
0.725 |
0.775 |
0.800 |
0.800 |
||
% Degradation (mean) |
0 |
9 |
7 |
8 |
26 |
26 |
27 |
26 |
28 |
||
O2Depletion (mg O2/l) |
R1 |
1.300 |
1.475 |
1.400 |
1.650 |
2.250 |
2.225 |
2.225 |
2.450 |
2.400 |
|
R2 |
1.250 |
1.425 |
1.400 |
1.550 |
1.900 |
2.025 |
2.225 |
2.200 |
2.300 |
||
% Degradation (mean) |
24 |
27 |
25 |
29 |
38 |
39 |
40 |
42 |
43 |
R1and R2= Replicates 1 and 2
The mean percentage degradation values are presented graphically in Figure 1. (see illustration)
The calculation of Theoretical Oxygen Demand (ThOD) values is given in Appendix 2.
The oxygen depletion of the inoculated control did not exceed 1.5 mg O2/l after 28 days, the residual oxygen concentration in the test bottles remained at 4.4 mg O2/l or greater in all test vessels and the difference between the extremes of replicate oxygen depletion values at the end of the test was less than 20% in all vessels thereby satisfying the validation criteria.
The test material attained 28% degradation after 28 days and, therefore, cannot be considered as readily biodegradable under the strict terms and conditions of OECD Guideline No 301D.
Variation in degradation rates on different sampling days was considered to be due to normal biological variation in respiration rates between control and test vessels.
The toxicity control attained 38% degradation after 14 days and 43% degradation after 28 days therefore confirming that the test material was not toxic to the sewage treatment micro-organisms used in the study.
The standard material, sodium benzoate, attained 64% degradation after 14 and 28 days thereby confirming the suitability of the test method and culture conditions.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- 1,3 Cyclohexanedione cannot be considered as readily biodegradable under the strict terms and conditions of OECD Guideline No. 301D
- Executive summary:
Introduction.A study was performed to assess the ready biodegradability of the test material in an aerobic aqueous medium. The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No 301D, "Ready Biodegradability; Closed Bottle Test” referenced as Method C.4-E of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC), and US EPA Fate, Transport, and Transformation Test Guidelines OPPTS 835.3110 Paragraph (o).
Methods.The test material, at a concentration of 1.5 mg/l, was exposed to sewage treatment micro-organisms with culture medium in sealed culture vessels in the dark at approximately 20C for 28 days. The degradation of the test material was assessed by the determination of the amount of oxygen consumed. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
Results.The test material attained 28% degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301D.
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