3-aminophenol

Endpoint summary

Description of key information

Toxicity to terrestial plants:

The comparative inhibition effect of test chemical on the germination rate of cucumis sativus was determined. Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 ± 1°C, the root elongation of each seed was measured to 1 mm. After 48 h of incubation in the dark at 25 +/- 1 deg C, the root elongation of each seed was measured to 1 mm. Root elongation half inhibition concentration (RC50) was investigated after 48 hours of exposure. RC50 for test chemical was reported to be 1000 mg/L.

Toxicity to birds:

Aim of this study was to determine the effect of test chemical on the growth of Redwing blackbird (Agelaius phoeniceus). Wild-trapped birds were preconditioned to captivity for 2 to 6 weeks and were usually dosed by gavage with solutions or suspensions of the test chemical in propylene glycol. LD50 values were calculated. The median lethal dose (LD50) value of the test chemical to Redwing blackbird (Agelaius phoeniceus) is determine to be 237 mg/kg. Based on the results, test chemical is moderately toxic to birds via oral exposure.

Additional information

Summarized result of toxicity of the chemical on the growth of terrestial plants and birds was studied by considering and collecting the data from various databases for target chemical. The studies are as follows:  

 

Toxicity to terrestrial plants:

Based on the various experimental data for the test chemical study have been reviewed to determine the mode of action of test chemical on the growth of terrestrial plants. The studies are as mentioned below:

 

In the first experimental key study from peer reviewed journal 2002, the comparative inhibition effect of test chemical on the germination rate of cucumis sativus was determined. Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 ± 1°C, the root elongation of each seed was measured to 1 mm. After 48 h of incubation in the dark at 25 +/- 1 deg C, the root elongation of each seed was measured to 1 mm. Root elongation half inhibition concentration (RC50) was investigated after 48 hours of exposure. RC50 for test chemical was reported to be 1000 mg/L.

 

First study was supported by the second study from peer reviewed journal 2001. Seed germination rate inhibition studies were conducted in Cucumis sativus for 48 hours with different concentrations of test chemical. Comparative inhibition effect of selected nitrogen-containing aromatics on germination rate of Cucumis sativus was investigated in which 15 seeds were germinated in solution of test chemical for 48 h in dark at 25 deg C; pH 6.24; 6 concentrations ranging from no effect to 100 percent inhibition concentration were tested and the germination rate was calculated. The GC50 (concentration that inhibited germination rate by 50 percent) value of test chemical in Cucumis sativus was observed to be 1203.9 mg/l.

 

Based on the both studies chemical toxicity value ranges from 1000 mg/l to 1203.9 mg/l.

 

Toxicity to birds:

Based on the various experimental data for the target chemical study have been reviewed to determine the mode of action of test chemical on the growth and other activity of birds. The studies are as mentioned below:

In the first experimental key study from peer reviewed journal 1983, toxicity was measured. Aim of this study was to determine the effect of test chemical on the growth of Redwing blackbird (Agelaius phoeniceus). Wild-trapped birds were preconditioned to captivity for 2 to 6 weeks and were usually dosed by gavage with solutions or suspensions of the test chemical in propylene glycol. LD50 values were calculated. The median lethal dose (LD50) value of the test chemical to Redwing blackbird (Agelaius phoeniceus) is determine to be 237 mg/kg. Based on the results, test chemical is moderately toxic to birds via oral exposure.

 

First study was supported by the second study from peer reviewed journal 1983. Study was conducted to determine the effect of test chemical on the growth of birds. Solutions or suspensions of the test chemical were prepared in propylene glycol. LD50 value of the test chemical to quail is determine to be 750 mg/kg. Based on the results, it was concluded that the test chemical is not toxic to birds via oral exposure.

 

Thus based on the both studies toxicity was ranges from 237 mg/l to 750 mg/l.