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EC number: 266-944-2 | CAS number: 67701-26-2 This substance is identified by SDA Substance Name: C12-C18 trialkyl glyceride and SDA Reporting Number: 16-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication meeting basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of structured medium- and long-chain triacylglycerols in diets with various levels of fat on body fat accumulation in rats
- Author:
- Matsuo, T. and Takeuchi, H.
- Year:
- 2 004
- Bibliographic source:
- British Journal of Nutrition 91:219–225
Materials and methods
- Principles of method if other than guideline:
- The effects of structured medium- and long-chain triacylglycerols (MLCT) in diets containing 50–200 g fat/kg on body fat accumulation were compared with those of long-chain triacylglycerols (LCT) in rats. The diets were fed to groups of 6 young adult male Wistar rats for 56 days.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- medium- and long-chain triacylglycerols
- IUPAC Name:
- medium- and long-chain triacylglycerols
- Details on test material:
- - Name of test material (as cited in study report): medium- and long-chain triacylglycerols (MLCT)
- Name of test material (as cited in study report): long-chain triacylglycerols (soybean-oil)
- Other: Soybean oil was used as the source of LCT, and was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). MLCT were prepared by transesterification of 200 g MCT and 800 g rapeseed oil, which were purchased from Nisshin Oil Mills (Tokyo, Japan).
- Composition of fatty acids and triacylglycerols of the test oils:
- LCT contained (g/100 g total fatty acids): linoleic acid 53, oleic acid 24, alpha-linolenic acid 8
- MLCT contained (g/100 g total fatty acids): oleic acid 50, linoleic acid 16, caprylic acid 14, capric acid 5
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Japan SLC, Inc., Shizuoka, Japan
- Age at study initiation: 4 weeks
- Housing: individually housed
- Diet: CE-2 (CLEA Japan, Tokyo, Japan), a commercial rodent diet from to the age of 4 weeks, ad libitum
- Water: ad libitum
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 24 ± 1
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): Half of the groups were fed diets containing LCT, and the other half were fed diets containing MLCT. - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 56 days
- Frequency of treatment:
- daily, 7 days/week
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
50, 100, 150 and 200 g/kg food (5, 10, 15 and 20%)
Basis:
nominal in diet
- Remarks:
- Doses / Concentrations:
4.09, 8.17, 12.26 and 16.34 g/kg bw/day
Basis:
other: mean dose value as calculated from the reported mean body weight and mean food intake values
- No. of animals per sex per dose:
- 6
- Control animals:
- no
Examinations
- Observations and examinations performed and frequency:
- On the final day, rats in each dietary group were fasted for 12 h, then killed by decapitation.
Blood was collected to obtain serum; liver and intra-abdominal adipose tissues (epididymal, perirenal and mesenteric) were quickly removed, weighed, and stored until analyses. Carcass samples were obtained by removing the head, tail and splanchnic tissues, and were stored at until analysis of carcass composition.
CAGE SIDE OBSERVATIONS: Yes
BODY WEIGHT: Yes
- Time schedule for examinations: daily
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study): Yes
- Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: Yes
FOOD EFFICIENCY:
Yes
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): No
OPHTHALMOSCOPIC EXAMINATION: No
HAEMATOLOGY: No
CLINICAL CHEMISTRY: Yes
- Parameters: serum glucose and triacylglycerols, serum insulin; further: liver total lipid, liver triacylglycerol concentrations, carcass fat and protein, hepatic capacities of citrate synthase and cytochrome oxidase
URINALYSIS: No
NEUROBEHAVIOURAL EXAMINATION: No - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes; at the end of the study, the rats were killed by decapitation, blood was collected to obtain serum, with the liver and intraabdominal adipose (epididymal, perirenal and mesenteric) tissues removed, weighed and stored at -40°C until analysis.
HISTOPATHOLOGY: No - Statistics:
- All data were analysed by a factorial ANOVA and post hoc Scheffe’s test. Differences were considered statistically significant at p<0.05.
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- non adverse
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Description (incidence and severity):
- non adverse
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- effects observed, treatment-related
- Description (incidence and severity):
- non adverse
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY
No effects observed.
BODY WEIGHT AND WEIGHT GAIN
In the LCT groups, final body weight and weight gain were significantly lower in rats fed 50 and 100 g fat/kg diet than in rats fed 150 and 200 g fat/kg diet. In the 150 and 200 g fat/kg diets groups, final body weight and weight gain were significantly lower in the MLCT groups than in the LCT groups
FOOD CONSUMPTION AND FOOD EFFICIENCY
Food intake was more suppressed by the feeding of a high-fat diet, and food efficiency increased significantly with increasing dietary fat both in the LCT and MLCT groups. In the 150 and 200 g fat/kg diet groups, food efficiency was significantly lower in the MLCT groups.
CLINICAL CHEMISTRY
Serum triacylglycerol concentration was significantly higher (P<0.05) in MLCT-fed rats, when compared to LCT-fed rats.
Serum glucose and insulin concentrations were significantly lower in the 50 g fat/kg diet group than in 100, 150, and 200 g fat/kg diet groups in rats fed LCT and MLCT diets, but the MLCT groups were not significantly different from the corresponding LCT dose groups.
Hepatic capacities of the citrate synthase and cytochrome oxidase were significantly higher in rats fed MLCT, when compared to LCT-fed rats.
ORGAN WEIGHTS
Liver weight and triacylglycerol content were not influenced by the levels of LCT and MLCT.
Liver triacylglycerol content was significantly increased in rats fed the MLCT diet, when compared to rats fed the 20% LCT/kg diet.
Perirenal adipose tissue weight and intra-abdominal adipose tissue weight was significantly (P<0.05) lower in the 15 and 20% MLCT dose groups, when compared to the 15 and 20% LCT dose groups.
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 200 000 mg/kg diet
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: based on clinical observations, body weight, food efficiency, liver weights and clinical serum chemistry.
- Dose descriptor:
- NOAEL
- Effect level:
- ca. 16 340 mg/kg bw/day (nominal)
- Based on:
- other: mean dose value as calculated from the reported mean body weight and mean food intake values
- Sex:
- male
- Basis for effect level:
- other: based on clinical observations, body weight, food efficiency, liver weights and clinical serum chemistry.
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
Table 2: Body weight, food intake and food efficiency in rats fed experimental diets
(Mean values for six rats per group)
|
Fat in experimental diets (g/kg) |
||||
50 |
100 |
150 |
200 |
||
Body weight (g) |
|
|
|
|
|
initial |
L |
87 |
88 |
88 |
89 |
ML |
88 |
88 |
88 |
88 |
|
final |
L |
273 |
275 |
293 |
297 |
ML |
272 |
272 |
272 |
278 |
|
gain |
L |
186 |
187 |
205 |
208 |
ML |
185 |
185 |
184 |
190 |
|
Food intake (g) |
|
|
|
|
|
|
L |
14.3 |
13.7 |
13.6 |
13.1 |
|
ML |
14.7 |
13.8 |
12.9 |
12.5 |
Food efficiency (mg/g) |
|
|
|
|
|
|
L |
236 |
248 |
274 |
289 |
|
ML |
229 |
245 |
259 |
276 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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