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EC number: 212-786-4 | CAS number: 869-24-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: General results were provided but experimental details and details on results were not provided. However, the based on the clear dose-response relationship, the results could be evaluated.
Data source
Reference
- Reference Type:
- publication
- Title:
- The mutagenicity of Dialkylaminoalkyl chlorides in a battery of short-term assays
- Author:
- Thompson CZ, Rinzel SM, Probst GS, McMahon RE
- Year:
- 1 981
- Bibliographic source:
- Environmental Mutagenesis 3:33-43 (1981)
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-chloroethyldiethylammonium chloride
- EC Number:
- 212-786-4
- EC Name:
- 2-chloroethyldiethylammonium chloride
- Cas Number:
- 869-24-9
- Molecular formula:
- C6H14ClN.ClH
- IUPAC Name:
- 2-chloroethyldiethylammonium chloride
- Details on test material:
- - Name of test material (as cited in study report): Diethylaminoethyl chloride
- Analytical purity: not provided. Since the test substance was obtained from a reliable supplier it is assumed that the purity was sufficiently high to use the results.
- Other: The test item was obtained from Aldrich Chemical Company, Inc. Milwaukee, Wisconsin, USA as hydrochloride salt.
Constituent 1
Method
- Target gene:
- tryptophan auxotrophy
Species / strainopen allclose all
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- WP2uvrA: ca. 1.75 - 225 µM
TA 1535: ca. 3.5 - 1800 µM
TA 100: ca. 3.5 - 1800 µM
Note: the molecular mass of DEC is 172.1 g/mol. Hence the concentrations ranged from about 0.3 µg/mL to about 310 µg/mL for WP2uvrA. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterile destilled water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- Details on test system and experimental conditions:
- According to Ames et al. (1975) modified as follows: Ten milliliter base layers o minimal agar were prepared and used on the same day of preparation. Ten serial twofold dilutions were made of each compound in sterile culture tubes to which were added 3 mL top agar and 0.1 mL of an overnight culture of the selected tester strain.
- Evaluation criteria:
- Not provided. In the publication, figures with error bars are presented in figure 4. Based on this information and the shape of hte dose-response relationship, the onset of mutagenicity was estimated.
- Statistics:
- not performed, but error bars were provided in the figure 4
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- starting at the lowest concentration, i.e., about 1.75 µM
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- starting at 7 µM
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- starting at about 112.5 µM
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
should be considered as potentially mutagenicic - Executive summary:
In the publication of Thompson et al. (1981), the AMES test was performed with DEC using E.coli strain WP2uvrA and S. typhimurium strains TA 1535 and TA 100. Cytotoxic concentrations were not reported. The results were not reported in detail but in one graph. Statistics was not performed. However, due to the clear dose response the information was sufficient to consider DEC as potentially mutagenicic in the AMES test.
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