Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-087-0 | CAS number: 133-06-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 March 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- GLP; The experiments were done according to ICI Group Environmental Laboratory standard operating procedure B168 version 03 in rooms 007,038 and 156. The method is based on that originally described by Bringman and Kuhn (Bringman G and Kuhn R (1980). Comparison of the toxicity thresholds of water pollutants to bacteria, algae and protozoa in the cell multiplication inhibition test. Water Research 14, 231-241, which has been modified by Slabbert (Slabbert J. L. (1986). Improved bacterial growth test for rapid water toxicity screening. Bulletin of Environmental Contamination and Toxicology 37, 565-569.).
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The experiments were performed according to IC Group Environmental Laboratory standard operating procedure B168 version 03 in rooms 007,038 and 156. The method is based on that originally described by Bringman and Kuhn (Bringman G and Kuhn R (1980). Comparison of the toxicity thresholds of water pollutants to bacteria, algae and protozoa in the cell multiplication inhibition test. Water Research 14, 231-241.) which has been modified by Slabbert (Slabbert J L (1986). Improved bacterial growth test for rapid water toxicity screening. Bulletin of Environmental Contamination and Toxicology 37, 565-569.). This procedure measures the degree of inhibition of growth of a pure culture of Pseudomonas putida during a six hour period when the cells are in logarithmic growth phase.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Physical state: white powder
- Analytical purity: 90.0 % (w/w)
- Batch number: sample leference RS 309/H, order number ERH 5167, test substance reference number T505
- Date of arrival: 8 June 1990 - Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- methanol
- Test organisms (inoculum):
- other: Pseudomonas putida, strain NCIB9494
- Total exposure duration:
- 6 h
- Test temperature:
- 25°C
- Nominal and measured concentrations:
- Captan, dissolved in methanol, at 0.056, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L in comparison with a growth medium only control, a solvent only (0.1% v/v methanol) control
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (DCP): 18 mg/L
- Key result
- Duration:
- 6 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.22 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Growth inhibition
- Key result
- Duration:
- 6 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.07 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Growth inhibition
- Results with reference substance (positive control):
- Growth inhibition: 98%
- Validity criteria fulfilled:
- not specified
- Remarks:
- The experiments were done according to ICI Group Environmental Laboratory standard. The method is based on that originally described by Bringman and Kuhn (Bringman G and Kuhn R (1980).
- Conclusions:
- The EC50 of captan to Pseudomonas putida is 0.22 mg/L. The EC10 was 0.07 mg/L. Based on the functional endpoints of soil microbial activity exposed via the soil matrix, no significant effects on the process mediated by soil micro-organisms would be expected.
- Executive summary:
The toxicity of captan technical to Pseudomonas putida was determined. The test doses were: blank, control, solvent control, nominal concentrations of 0.056, 0.10 , 0.18, 0.32, 0.56 and 1.0 mg/1 and a reference concentration of 3,5-dichlorophenol (18 mg/L), 6 hours shaken.
The test species was Pseudomonas putida, strain NCIB9494. Percentage inhibition was plotted against log10concentrations and the EC10 and EC50 values were read directly from the graph. These were the concentrations of captan technical which covered 10% and 50% inhibition of growth respectively. The EC10 and EC50 values were:
EC10 = 0.07 mg/L nominal
EC50 = 0.22 mg/L nominal
Reference
The degree of inhibition of growth compared with that of the controls was calculated as follows:
% inhibition = {1 - [ mean optical density of test flask - chemical control value* ] } x 100 / [ mean optical density of control flask - blank control value ]
Percentage inhibition was plotted against log10concentrations and the EC10 and EC50 values were read directly from the graph. These were the concentrations of captan technical which covered 10% and 50% inhibition of growth respectively. The EC10 and EC50 values were:
EC10 = 0.07 mg/L nominal
EC50 = 0.22 mg/L nominal
Table 6.3.4-2 Summary of effects of captan on Pseudomonas putida
Captan concentration (mg/L)1 | Mean optical density minus value for uninoculated solution | Growth inhibition (%) |
Control2 | 0.6293 | - |
Solvent only | 0.6958 | 0 |
0.056 | 0.6362 | 0 |
0.1 | 0.4792 | 24 |
0.18 | 0.3765 | 40 |
0.32 | 0.2122 | 66 |
0.56 | 0.0912 | 86 |
1.0 | 0.0589 | 91 |
3,5-DCP | 0.0131 | 98 |
1Nominal concentrations
2Growth medium only
Description of key information
Toxicity to soil microorganisms ( Pseudomonas putida): The EC50 of captan to Pseudomonas putida is 0.22 mg/L carried out in aqueous solution. No significant effects on the process mediated by soil micro-organisms would be expected.
Key value for chemical safety assessment
Additional information
The EC50 and EC10 of captan to Pseudomonas putida, a commonly occurring soil and water bacterium, was 0.22 and 0.07 mg/L, respectively. The P. putida test was carried out in the water phase, assuming exposure is higher relative to bacteria in soil. Based on the functional endpoints of soil microbial activity exposed via the soil matrix, no significant effects on the process mediated by soil micro-organisms would be expected following the recommended use of captan.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.