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EC number: 248-670-5 | CAS number: 27816-23-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Remarks:
- HET-CAM Test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study report data
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: HET-CAM Test
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance to Regulation (EC) No 1272/2008 on classification, labelling and packaging (CLP) of substances and mixtures
- Principles of method if other than guideline:
- With the HET-CAM the irritation property of a test item after contact with mucous membranes or the eye can be determined based on the occurrence of alterations at the CAM and the adjacent capillary system of fertilized and incubated chicken eggs.
The chorioallantoic membrane (CAM) is a vital vascular membrane with a closed blood vessel system. After application of a test item on the membrane the underlying blood vessels could become damaged. In particular three events are observed:
- hemorrhage (H) (bleeding out from the vessels of the vascularized CAM)
- vessel lysis (L) (disappearance of small vessels)
- protein coagulation (C) (intravasal or extravasal)
Two different methods can be used depending on the transparency of the test item, the reaction-time method or the end-point assessment. Since 2-methoxyethyl 2-cyanoacrylate was shown to polymerize and harden on the CAM, the end-point assessment was applied.
The study procedures were based on the following document:
- The Ocular Toxicity Working Group (OTWG) of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and the National Interagency Centre for the Evaluation of Alternative Toxicological Methods (NICEATM), Background Review Document (BRD): current status of in vitro test methods for identifying ocular corrosives and severe irritants: The Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) test method, March 2006.
- Steiling et al. (1999) The HET-CAM, a useful in vitro assay for assessing the eye irritation properties of cosmetic formulations and ingredients. Toxicology in Vitro 13, 375-384 (1) - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-methoxyethyl 2-cyanoacrylate
- EC Number:
- 248-670-5
- EC Name:
- 2-methoxyethyl 2-cyanoacrylate
- Cas Number:
- 27816-23-5
- Molecular formula:
- C7H9NO3
- IUPAC Name:
- 2-methoxyethyl 2-cyanoprop-2-enoate
- Test material form:
- liquid
- Details on test material:
- 2-methoxyethyl 2-cyanoacrylate
Test Item name: SAT 130032
Batch: #L2PW120143
Constituent 1
Test animals / tissue source
- Species:
- other: HET-CAM
- Strain:
- other: not applicable
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent positive control
- Amount / concentration applied:
- 300 µL were topically applied to the CAM of 6 individual eggs each.
- Duration of treatment / exposure:
- 300 s
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- On day 10, after nine days incubation, the eggs were candled to check the correct development of the fertilized egg as well as to visualise air chamber position of individual eggs. Along the marked air chamber the eggshell was carefully sawed and the CAM was uncovered. Only eggs with a marked blood vessel system were used for the test. Eggs with a non-marked vessel system were discarded.
300 μL of the test item and the same amount of Texapon ASV Spezial (5 %) as a reference were topically applied to the CAM of 6 individual eggs each. The eggs were exposed for 300 seconds to the test item and the reference compound. Results for the three test method endpoints were evaluated separately for each egg and the S-score was calculated. The test item was classified by means of the strongest reaction type.
Classification scheme of the HET-CAM End-Point Assessement:
S [sum of single value] Evaluation
0 – 5 slightly irritating
6 – 12 moderately irritating
13 – 15 irritating
16 – 18 severely irritating (R 41)
Results and discussion
In vitro
Results
- Irritation parameter:
- other: HET-CAM S-score
- Run / experiment:
- mean 300 s treatment
- Value:
- 2
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Egg No. | Severity Score of Haemorrhage | Severity Score of Lysis | Severity Score of Coagulation | |
Test Item | 1 | 1* | 0 | 0 |
2 | 1 | 0 | 0 | |
3 | 1 | 0 | 0 | |
4 | 1* | 0 | 0 | |
5 | 2* | 0 | 0 | |
6 | 2 | 0 | 0 |
*When lifting and removing of the test item from the CAM, a vessel was injured (mechanical damage)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Criteria used for interpretation of results: ICCVAM
- Conclusions:
- In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item possesses a moderate irritating potential to mucous membranes or eyes.
- Executive summary:
The irritation potential of 2 -methoxyethyl 2 -cyanoacrylate to eyes and mucous membranes was evaluated in the HET-CAM (Hen's Egg Test – Chorioallantoic Membrane) by analysing specific effects to the membrane and/or vessels of the CAM of 9 day incubated chicken eggs. The specific sensitivity of the batch of eggs could be shown by means of the reference item.
2 -Methoxyethyl 2 -cyanoacrylate was tested undiluted. After application, the test item started to harden within 30 seconds on the CAM and became turbid. Since the CAM became invisible due to the test item's hardening process effects were evalutated using the endpoint method (EPM). After completion of the first 30 seconds of exposure of test item to the CAM according to the EPM, the test item could not be rinsed off. Therefore, the exposure period was prolonged to 5 minutes to have the hardening process completed. Afterwards, the hardened test item could be carefully lifted and removed as one piece.
The occurrence of reactions on the blood vessels under the CAM was monitored after removing of the test item.
The individual test results were interpreted in comparison to the effects of the reference item Texapon ASV Spezial which is defined to be a moderately irritating compound to the rabbit eye in vivo. The dilutions of Texapon ASV Spezial with 0.5%, 1%, and 5% active substance (AS) in deionised water were used as reference for the scoring of the effects on the CAM caused by the test item. After 5 minutes of treatment with Texapon ASV Spezial dilutions, the 0.5% solution demonstrated weak effects, the 1% solution moderate effects, and the 5% solution strong effects on the CAM.
Coagulation or lysis were not observed after exposure of the test item to the CAM, but slight to moderate haemorrhaging effects were visible. However, in three of six eggs lifting resulted in mechanical damage of vessels. By also considering those results where mechanical damage occured, the highest summed up score was 8 for haemorrhage. Consequently, the test item is classified as moderately irritating to mucous membranes or eyes.
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