3,5-xylidine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

Study on behalf of competent authorities of Japan,

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

not specified

Specific details on test material used for the study:

Purity: 98.5%
Lot: DU01
Code: K-1026
liquid

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

liquid volume: 300 mL
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): derived from a mixture of source
Fushikogawa Treatment Plant (Sapporo City, Hokkaido)
Nakahama Treatment Plant (Osaka City, Osaka Prefecture)
Kitakami River (Ishinomaki City, Miyagi Prefecture)
Yoshino River (Tokushima City, Tokushima Prefecture)
Hiroshima Bay (Hiroshima City, Hiroshima Prefecture)
Fukashiba Treatment Plant (Kashima-gun, Ibaraki)
Ochiai Treatment Plant (Shinjuku-ku, Tokyo)
Shinano River (Nishikanbara-gun, Niigata)
Lake Biwa (Otsu City, Shiga Prefecture)
Dokai Bay (Kitakyushu City, Fukuoka Prefecture)

- Method of cultivation: The filtrate of each of the sludge collected in the above-mentioned regions was mixed with 54 filtrate of the old activated sludge that had been used for the test. The pH was adjusted to 7.0 ± 1.0 and aeration in an incubator
- Water filtered: yes
- The concentration of suspended substance in the activated sludge was 5800 mg/L
- pH adjusted to 7.0

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

BOD

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

other: HPLC

Reference substance:

aniline

Parameter:

other: HPLC

Value:

0

Sampling time:

4 wk

Parameter:

% degradation (TOC removal)

Value:

3

Sampling time:

4 wk

Remarks on result:

other: 3 - 4 %

Parameter:

% degradation (O2 consumption)

Remarks:

BOD

Value:

3

Sampling time:

4 wk

Remarks on result:

other: 1 - 5 %

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance was not degraded by microorganisms under the test conditions.

Executive summary:

The test substance 3,5-xylidine was assessed in a biodegaradtion study according to OECD Guildeine 301C (modified MITI Test (I)).

The test substance was incubated in activated sludge for 28 days at a concentration of 100 mg/L with a liquid volume of 300 mL at 25 +/- 1°C. The measurement of the biochemical oxygen demand (BOD) was performed by a closed oxgen consumption measurment device and the quantitative analysis was performed by the analysis method of total organic carbon (TOC). Quantitative analysis of the test substance was done by high-preformance liquid chromatography (HPLC).

The test results were as follows:

 

BOD degradation rate: 1%, 5%, 0%, 

TOC degradation rate: 4%, 3%, 3%

Test substance degradation (HPLC): 0%, 0%, 0%, 

 

The tes substance was not degraded by microorganisms under the conditions of the test.

Description of key information

OECD 301 C, BOD degradation rate: 1%, 5%, 0%; TOC degradation rate: 4%, 3%, 3%; HPLC: 0%, 0%, 0% after 28 days

 

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater