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EC number: 255-980-4 | CAS number: 42872-29-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting: 2009-06-16 - experimental completion: 2009-06-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to international guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-(1-cyanoethyl)benzoyl chloride
- EC Number:
- 255-980-4
- EC Name:
- 3-(1-cyanoethyl)benzoyl chloride
- Cas Number:
- 42872-29-7
- Molecular formula:
- C10H8ClNO
- IUPAC Name:
- 3-(1-cyanoethyl)benzoyl chloride
- Details on test material:
- TEST ITEM: Ketoprofen I CFPPN
Batch number: F-PPR-080059
CAS RN: 42872-29-7
Chemical name: 3-(1-Cyanoethyl)benzoyl chloride
Content: 95.0%
Appearance: Brown liquid
Sum formula: C10H8ClNO
Molecular weight: 193.63 g/mol
Water solubility: Hydrolyses at 20 °C
Expiry date: 2009-08-12
Recommended storage: Refrigerator, 7 ± 2 °C, protected from moisture and light
Storage at test facility: 6 ± 2 °C, protected from moisture and light
Retention of test item: At least 1 g has been retained.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Stock solutions of 100 mg/L of each standard were prepared in methanol. A mixed standard of 4.0 mg/L was prepared from these
stock solutions in mobile phase, diluted with mobile phase (6 concentrations (Diluted level of the saturated concentration: 1:1 -1:2-1:4-1:8-1:16)), and used for calibration.
- Sampling method: Analytical evaluation of various concentrations of Ketoprofen, active ingredient of Ketoprofen / CFPPN, and its metabolite Capril was carried out via HPLC on a reversed-phase column using external standards. Analysis was performed in gradient mode with a Diode Array Detector.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Stock solution: The stock solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
Dispersion treatment: The stock solution was shaken with 20 rpm for 24 h (rotating shaker 3040, GFL). Undissolved particles were removed by centrifugation with 3000 rpm for 20 min at room temperature.
Test dilutions of the saturated solution: 1:1 -1:2-1:4-1:8-1:16 (dilution factor: 2), corresponding to the nominal test item concentrations of 100 - 50.0 - 25.0 - 12.5 - 6.25 mg test item/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Origin: lnstitut fOr Wasser-, Boden- und Lufthygiene (WaBolu)
Breeder: DR.U.NOACK-LABORATORIEN, Kathe-Paulus-Str. 1, D-31157 Sarstedt, Germany
Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µE.m^-2/s .
Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaC03/L.
Feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata, Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 10^6 cells/ml.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48h
Test conditions
- Hardness:
- Hardness of the dilution water: 265 mg CaC03/L
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- Additionally a pH-control was tested. Therefore, 4 replicates of the highest test concentration was adjusted to a pH in the range of 7.0 ± 0.2 by addition of 1 M NaOH.
- Dissolved oxygen:
- The dissolved oxygen concentration was 3 mg/L.
- Nominal and measured concentrations:
- The nominal test item concentrations: 100 - 50.0 - 25.0 - 12.5 - 6.25 mg/l
- Details on test conditions:
- Test vessels: Glass beakers (4 cm ID x 7 cm H), 50 ml capacity
Test volume per vessel: 20 ml
Number of study organisms: 20 animals, divided into 4 parallel samples, each with 5 animals per test concentration, pH control and control
Age of test organisms: 2 to 24 h old daphnids were used for the definitive study. They were obtained by removing the mother animals twice within 22 h.
Acclimatization: At least 2 h in dilution water
Illumination: Diffuse light, illumination strength max. 20 IJE/m/s
Photoperiod: 16/8 h light/dark cycle
Feeding: The daphnids were not fed during the study. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: CI 50.0- 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Cl: 50.0- 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- 67.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 67.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24h. The EC50-value with 95 % confidence interval (Cl) was calculated by sigmoidal dose-response regression to be:
EC50: 1.66 mg/L (Cl 1.50- 1.83 mg/L)
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202. - Reported statistics and error estimates:
- The EC100-values after 24 and 48 h, the NOEC and the LOEC were deduced directly from the dose-response-relationships without mathematical calculation.
EC10-values were calculated by sigmoidal dose-response regression. Since only 0 % and 100 % immobilization occurred, the EC50-value was determined by calculating the geometric mean of the highest concentration causing no immobilization and the lowest concentration causing 100 % immobilization. As confidence limits for the EC50 the highest concentration causing no immobility and the lowest concentration causing 100 % immobility were used, because only 0% and 100 % mortality occurred within the tested concentration range. The concentration effect relationships are shown graphically.
The EC50 for the of the reference test were calculated by sigmoidal dose- response regression. The confidence interval for the EC50 of the reference test was calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study Ketoprofen/CFPPN was found to be toxic to Daphnia magna after 48h at concentrations of 100 mg/L (LOEC48 h). The NOEC after 48 hours was 50.0 mg/L. The EC50 (48h) was calculated to be 70.7 mg/L (Cl: 50.0- 100 mg/L). All effect values are given based on the nominal concentrations of the test item Ketoprofen/CFPPN.
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