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EC number: 238-914-9 | CAS number: 14852-17-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- Acute Immobilization Study of test material on Daphnia magna in a Static System for 48 hrs. Test performed according to the OECD guideline 202.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 180 mg of CaCO3
- Test temperature:
- 20±2
- pH:
- 7.4
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 12.5 - < 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: The EC50 > 12.5 mg/L but < 25 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be > 12.5 mg/L but < 25 mg/L mg/L.
- Executive summary:
The effect of test chemical was studied by taking the daphnia magna as the animal model. Daphnia sp., Acute Immobilization Test was conducted according to OECD Guideline 202. The stock solution was prepared by dissolving test chemical of 200 mg in 200ml of ADAMS media. The test concentration selected for the exposure was 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively. The study was conducted in static conditions for the period of 48 hours and observed for any occurance immobilization. The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Reference
9.1. Immobility:
Nominal |
Number |
of |
Immobility |
|
Concentration |
Daphnids |
0 h |
24 h |
48 h |
(mg/l) |
|
|
|
|
Control |
10 |
- |
- |
- |
1.5625 |
10 |
- |
- |
- |
3.125 |
10 |
- |
- |
- |
6.25 |
10 |
- |
- |
- |
12.5 |
10 |
- |
1 |
1 |
25 |
10 |
- |
3 |
6 |
9.2. pH and oxygen content:
Nominal Concentration Number of |
pH(mg/L) |
|
|
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
7.4 |
7.2 |
1.5625 |
10 |
7.7 |
7.1 |
3.125 |
10 |
7.8 |
7.2 |
6.25 |
10 |
7.5 |
7.7 |
12.5 |
10 |
7.7 |
7.4 |
25 |
10 |
7.7 |
7.4 |
Nominal Concentration Number of |
DO(mg/L) |
|
|
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
8.6 |
6.9 |
1.5625 |
10 |
8.7 |
6.9 |
3.125 |
10 |
8.4 |
6.3 |
6.25 |
10 |
8.3 |
6.4 |
12.5 |
10 |
8.2 |
6.2 |
25 |
10 |
7.3 |
6.5 |
9.3. Test temperature:
Nominal Concentration Number of |
Temparature |
||
(mg/l) |
daphnids |
0h |
48h |
Control |
10 |
21.4 |
21.1 |
1.5625 |
10 |
21.5 |
21.1 |
3.125 |
10 |
21.5 |
21.2 |
6.25 |
10 |
21.5 |
21.2 |
12.5 |
10 |
21.5 |
21.3 |
25 |
10 |
21.5 |
21.4 |
Description of key information
Short-term toxicity to aquatic invertebrates:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium butanedioate. The test substance was soluble in ADaMs’ media. The in-house water solubility of test substance in milli Q water was found to be 5509.96 mg/L. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively.
The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 574 mg/L
Additional information
Short-term toxicity to aquatic invertebrates:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium butanedioate. The test substance was soluble in ADaMs’ media. The in-house water solubility of test substance in milli Q water was found to be 5509.96 mg/L. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively. The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
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