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EC number: 206-566-7 | CAS number: 354-64-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-04-10 to 1992-07-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study is performed according to Guideline 471, under GLP and is well reported.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pentafluoroiodoethane
- EC Number:
- 206-566-7
- EC Name:
- Pentafluoroiodoethane
- Cas Number:
- 354-64-3
- Molecular formula:
- C2F5I
- IUPAC Name:
- 1,1,1,2,2-pentafluoro-2-iodoethane
- Details on test material:
- - Name of test material (as cited in study report): Pentafluorethyljodid
- Molecular formula (if other than submission substance): C2 F5 J
- Physical state: colourless gas
- Analytical purity: about 97 -98 %
- Impurities (identity and concentrations): Tetrafluorethylene; about 1.19 % to 1.6 %
- Purity test date: 1992-04-07
- Lot/batch No.: 4847 (1286524)
- Expiration date of the lot/batch: August 1993
- Radiochemical purity (if radiolabelling):
- Storage condition of test material: dark at room temperature
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced liver S9 homogenate mixture
- Test concentrations with justification for top dose:
- 0.8 vol. %/plate to 20.1 vol. %/Plate proved to be not toxic to the bacteraial strains.
For mutagenicity testing 18.1 vol %/plate was chosen as the highest dose in the first main experiment. - Vehicle / solvent:
- air
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9 mix
Migrated to IUCLID6: TA 100, TA 1535
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9 mix
Migrated to IUCLID6: TA 1537
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without S9 mix
Migrated to IUCLID6: TA 98, TA 1538
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene for all 5 tester strains
- Remarks:
- with S9 mix
- Details on test system and experimental conditions:
- Preliminary experiment was performed in all tester strains using three plates per dose. In combination with the main experiment, toxicity testing was performed as follows:
the same doses of the test compound as in the main experiment were tested with 0.1 mL of 10-6 dilution of the overnight culture of TA 100 and plate with histidine and biotin rich agar (3 plates per dose). The solvent control is compared with the number of colonies per plate in presence of the test compound. Results are given as a ration of these values (= surviving fraction).
For the mutagenicity testing the prepared liquid top agar is poured into a petridish with minimal agar. In this way pretreated plates are arranged by doses and placed open in an exsictor which is connected to a gas sampler. The exsicator is inflated with gas-air mixture by an air calibrated rotameter for approx. 10 minutes. The inflated gas-samplers are sent to the laboratory to analyze the exact concentration of gas (in the gas-air mixture). After incubation for 48 hours at approx. 37°C in the dark colonies (his+ revertants) are counted. Four independent experiments were performed. - Evaluation criteria:
- The article is classified mutagenic if either of the following conditions under a) and b) is achieved:
a) a test article produces at least a 2-fold increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control at complete bacterial background lawn.
b) a test acticle induces a dose-related increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control in at least two to three concentrations of the test article at complete bacterial background lawn.
The test results must be reproducble.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this Ames test it can be stated that the test substance is mutagenic with and without metabolic activation.
- Executive summary:
The test compound was tested for mutagenicity with the tester strains S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100.
The mutagenicity studies were coducted with and without S9 mix derived from Aroclor induced rat liver homogenate. A dose range of 5 or 6 different doses from 0.8 vol. %/plate to 38.6 vol. %/plate was used.
The test substance proved to be not toxic to the bacterial strains. 38.6 vol. %/plate was tested as top dose level for mutagenicity study.
In the absence of the metabolic activation system, the test compond gave a dose dependent increase in the number of revertant colonies with the bacterial strain TA 1537. In the presence of metabolic activation, treatment of cells with the test compound results in relevant increases in the number of revertant colonies with the Salmonella strain TA 1537.
Based on the results of this test it can be stated that the test substance is mutagenic with and without metabolic activation.
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