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EC number: 229-494-8 | CAS number: 6574-99-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-02-11 - 1997-02-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Directive 96/54/EC, Part B.1 tris "Acute Toxicity-Oral, Acute Toxic Class Method"
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 3,4-dichlorobenzonitrile
- EC Number:
- 229-494-8
- EC Name:
- 3,4-dichlorobenzonitrile
- Cas Number:
- 6574-99-8
- Molecular formula:
- C7H3Cl2N
- IUPAC Name:
- 3,4-dichlorobenzonitrile
- Test material form:
- solid: bulk
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, Sulzfeld, Germany.
- Age at study initiation: approx. 6 weeks
- Weight at study initiation: within +/- 20 % of the sex mean
- Fasting period before study: food was withheld overnight prior to dosing until approximately 3-4 hours after administration of the test substance.
- Housing: group housing of 3 animals per sex per cage in labelled polycarbonate cages containing purified sawdust as bedding material (Woody SPF, supplied by B.M.I, Helmond, The Netherlands)
- Diet (e.g. ad libitum): free access to standard pelleted laboratory animal diet (from Carfil Quality BVBA, Oud-Turnhout, Belgium)
- Water (e.g. ad libitum): free access to tap-water
- Acclimation period: at least 5 days before start of treatment under laboratory conditions.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 50
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): lighting was 12 hours artificial flourescent light and 12 hours dark per day
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- - Method: Oral gavage, using a stainless steel stomach tube.
- Frequency: Once, on day 1.
- Animals: 3 animals of the same sex per dose group. - Doses:
- 200 mg/kg (females), 200 mg/kg (males) and 2000 mg/kg (females)
- No. of animals per sex per dose:
- three
- Control animals:
- no
- Details on study design:
- OBSERVATIONS:
- Mortality/Viability: Twice daily. The time of death was recorded as precisely as possible
- Body weights: Days 1 (pre-administration), 8 and 15 and at death (if found death after day 1).
- Clinical signs: At periodic intervals on the day of dosing (day 1) and once daily thereafter, until day 15. The time of onset, degree and duration were recorded.
- Necropsy of survivors performed: yes - Statistics:
- not performed
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 200 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 200 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All femals dosed at 2000 mg/kg b.w. were found dead within 24 hours of dosing.
- Clinical signs:
- other: 200 mg/kg: Hunched posture, uncoordinated movements. 2000 mg/kg: Lethargy, hunched posture, uncoordinated movements, piloerection. The surviving animals had recovered from symptoms between days 2 and 3. The surviving animals had recovered from the sympto
- Gross pathology:
- No abnormalities were found at macroscopic post mortem examination of the animals.
Any other information on results incl. tables
The test substance was ranked within LD50 values ranges of 0 - 25, 25 - 200 or 200 - 2000 mg/kg b.w. or as exceeding 2000 mg/kg b.w.
No statistical analysis was performed (The method used is not intended to allow the calculation of a percise LD50 value).
The results were evaluated according to the EEC criteria for classification and labelling requirements for dangerous substances and preparations (Guidelines in Commission Directive 93/21/EEC, 27th april 1993).
Table 1: Body weights of test groups.
Group / Sex |
Animal |
Day 1 [g] |
Day 2 [g] |
Day 8 [g] |
Day 15 [g] |
Group 1 / females |
1 |
126 |
|
179 |
202 |
2 |
131 |
|
171 |
195 |
|
3 |
122 |
|
161 |
170 |
|
Mean |
126 |
|
170 |
189 |
|
St. Dev. |
5 |
|
9 |
17 |
|
N |
3 |
|
3 |
3 |
|
Group 2 / males |
4 |
185 |
|
249 |
283 |
5 |
194 |
|
277 |
309 |
|
6 |
186 |
|
239 |
277 |
|
Mean |
188 |
|
255 |
290 |
|
St. Dev. |
5 |
|
20 |
17 |
|
N |
3 |
|
3 |
3 |
|
Group 3 / females |
7 |
138 |
137 |
- |
- |
8 |
148 |
147 |
- |
- |
|
9 |
143 |
137 |
- |
- |
|
Mean |
143 |
|
- |
- |
|
St. Dev. |
5 |
|
- |
- |
|
N |
3 |
|
0 |
0 |
Table 2: Observed clinical signs of test group 1 (females, 200 mg/kg)
Test day |
1 |
1 |
1 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
||
Time after treatment. hours |
0 |
2 |
4 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Animal number |
Signs |
max. grade |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1 |
gait/motility: uncoordinated movements |
(3) |
- |
1 |
2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
2 |
gait/motility: uncoordinated movements |
(3) |
- |
1 |
1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
3 |
Posture: Hunched posture
gait/motility: uncoordinated movements |
(1)
(3) |
-
- |
-
2 |
-
2 |
1
1 |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
-
- |
Table 3: Observed clinical signs of test group 2 (males, 200 mg/kg)
Test day |
1 |
1 |
1 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
||
Time after treatment. hours |
0 |
2 |
4 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Animal number |
Signs |
max. grade |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4 |
gait/motility: uncoordinated movements |
(3) |
- |
1 |
2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
5 |
gait/motility: uncoordinated movements |
(3) |
- |
1 |
2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
6 |
gait/motility: uncoordinated movements |
(3) |
- |
1 |
2 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Table 4: Observed clinical signs of test group 3 (females, 2000 mg/kg).
Test day |
1 |
1 |
1 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
||
Time after treatment. hours |
0 |
2 |
4 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Animal number |
Signs |
max. grade |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
7 |
Behavior: Lethargy
Posture: Hunched posture
gait/motility: uncoordinated movements |
(3)
(1)
(3)
|
-
-
- |
-
-
2 |
1
1
2 |
+
+
+ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
8 |
Posture: Hunched posture
gait/motility: uncoordinated movements |
(1)
(3) |
-
-
|
-
2 |
1
2 |
+
+ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
9 |
Posture: Hunched posture
gait/motility: uncoordinated movements
Skin /FUR / Plumage: Piloerection |
(1)
(3)
(1) |
-
-
- |
-
2
1 |
1
2
1 |
+
+
+ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The oral LD50 value of 3,4-Dichlorobenzonitrile in Wistar rats was established to be within the range of 200-2000 mg/kg body weight.
According to the EEC criteria for classification and labelling, 3,4-Dichlorobenzonitrile must be classified as harmful if swallowed.
Based on the test result and observation, it can be expected that the classificatoin is also suitable for H302 where 300mg/kg is the threshold. - Executive summary:
The study was carried out based on the guidelines described by oral gavage to three femal Wister rats at 200 mg/kg body weight. In a stepwise procedure additional groups of animals were dosed at 200 (males) and 2000 (females) mg/kg body weight. All animals were subjected to daily observations and weekly determination of body weight. Macroscopic examination was performed on the day of death or after terminal sacrifice 8 (day 15).
All three animals dosed at 2000 mg/kg b.w. were found dead within 24 hours of dosing. The surviving animals recovered from all clinical signs observed between days 2 and 3. The body weight gain shown by the surviving animals over the study period was considered to be normal. No abnormalities were found at macroscopic post mortem examination of the animals.
The oral LD50 value of 3,4 -Dihchlorobenzonitrile in Wistar rats was established to be within the range of 200 -2000 mg/kg body weight.
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