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EC number: 270-418-8 | CAS number: 68439-75-8 This substance is identified by SDA Substance Name: C12-C18 dialkyl methyl amine and SDA Reporting Number: 16-043-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2008-02-11 to 2008-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- no data
- Vehicle:
- no
- Details on test solutions:
- Since the substance is not soluble in water, Water Accomodated Fractions (WAF) approach has been followed.
WAFs are prepared by adding carefully measured amounts of the substance directly to water and by mixing for 72 h to achieve an equilibrated concentration of the components in the aqueous phase. After mixing, solutions were filtered to obtain limpid solutions. No water-miscible solvents were used. Care is taken to avoid the formation of emulsions.
Excepted for the lower loading rates, WAFs are prepared individually and not by serial dilution of a single stock WAF. Measured amounts of multi-component substances are added. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source: Culture Centre of Algae and Protozoa (Ambleside, UK)
ACCLIMATION
Culturing media and conditions:
Two flasks, each containing approximately 100 mL of axenic stock culture of algae are incubated at 23 ± 1 °C under lighting (photoperiod: 16 hours of illumination, 8 hours of darkness), slowly continuously shaken. These stock cultures are renewed every week, using two new cultures.
The quality of the stock culture was verified for the absence of micro-organisms and deformed cells under microscopic observation before use.
Three days before the beginning of the study two pre-cultures were prepared by inoculating each stock suspension of algae (5 mL) into sterile dilution water (500 mL). The pre-cultures were incubated under the same conditions as those used for the stock cultures. Only one of the two pre-cultures was used to inoculate the test flasks; the second one was to be used only if the first one was damaged.
At the beginning of the test, the cell concentration of the pre-culture was determined. The result was used to calculate the volume to be introduced into each test flask in order to get an initial cell concentration of 104 cells/mL.
The cell density of the pre-culture was about 6.587 x 106 cells/mL. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- no data
- Test temperature:
- 23 ± 1 °C
- pH:
- 7.82 - 9.97
- Dissolved oxygen:
- 8.2 - 10.5 mg O2/L
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 0.01 - 0.05 - 0.1 - 0.5 and 1 mg/L
All measured concentrations were found to be below the detection limit of the analytical method (0.73 µg/L). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 120 mL glass bottles stoppered with cellulose bungs
- Filling volume: 50 mL
- Incubation performed in a phytoculture cabinet
- Triplicates in each treatment and 6 replicates for the control group
OTHER TEST CONDITIONS
- Illumination: constantly illuminated by 8 fluorescent tubes between 6,000 and 10,000 lux
- Continuously shaken with a rotation at 20 rpm - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL = 0.08-4.7 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Solutions were found to be clear, colourless over the period of the test. No precipitation was observed at the end of the test.
Microscopic observation confirmed that the algae appeared normal at the end of the test. - Results with reference substance (positive control):
- Sodium dichromate: ErC50: 0.60 to 1.03 mg/L EbC50: 0.20 to 0.75 mg/L
- Validity criteria fulfilled:
- yes
- Remarks:
- see conclusions
- Conclusions:
- Validity criteria were fulfilled:
- The biomass in the control cultures has increased exponentially by a factor higher than 16 within the 72-hour test period. This corresponds to a specific growth rate of 0.92 day-1.
- The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures did not exceed 35%. This criterion applies to the mean value of coefficients of variation calculated for replicate control cultures.
- The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures did not exceed 7%. - Executive summary:
Acute toxicity of dicocomethylamine to Pseudokirchnerella subcapitata has been examinated through OECD Guideline 201 and GLP requirements.
Pseudokirchnerella subcapitata were exposed for 72 hours to an aqueous solution of dicocomethylamine
at nominal concentrations of 0.01 - 0.05 - 0.1 - 0.5 and 1 mg/L.ErC 50 - 72h, based on growth rate, was determined to be of 0.7 mg/L, and EbC 50 - 72h, based on biomass, was determibed to be of 0.26 mg/L.
Reference
Description of key information
Based on a study according to OECD 201 guidelines EC50 for algae is 0.7 mg/L.
A further algae study with Amines, C12-18-alkylmethyl is recently performed and the proposed draft report is planned to receive at the end of February 2023. The final report is expected to be available at the end of March 2023.
This information will be submitted a little bit later based on ECHA communication/decision number CCH-D-2114585933-34-01/F.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.7 mg/L
Additional information
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