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EC number: 600-026-8 | CAS number: 1000817-22-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analysed.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Fresh stock solutions were prepared daily and each concentration prepared separately by directly adding test substance to test medium and stirring for approximately 1 hour at 20 ± 2 °C. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Clone: Daphnia magna STRAUS 1820
- Source: supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: none
CULTURE CONDITIONS
- Culture media: synthetic fresh water (Elendt M4) prepared according to OECD 202
- Diet: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding: Daily
- Renewal of the culture water: Twice weekly
- Temperature: 20 ± 1 °C
- Photoperiod: 16 hours light, 8 hours dark - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- 9.0 - 9.1 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 0.56, 1.0, 1.8, 3.2, 5.6, 10 mg/L
Measured test concentrations: (based on total organic carbon) 0,LOQ = limit of quantification (1.2 mg/L) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test vessels consist of cylinders of transparent glass, 6 cm diameter, 10 cm high, with a stainless-steel grid bottom of 0.2 mm x 0.2 mm mesh. The test vessels were suspended in overflow chambers and were filled with test solution to a height of 5 cm.
- Type of flow-through (e.g. peristaltic or proportional diluter): peristaltic pump
- Renewal rate of test solution (frequency/flow rate): The flow rate per replicate was 0.6 mL/min.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater (Elendt M4)
- Alkalinity: 0.8 - 1.0 mmol/L
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: Measurement of the pH and oxygen content at the start of the exposure and at the end of the exposure in each replicate of all test concentrations and the control. Measurement of temperature continuously during the whole period in a separate vessel filled with water proximal to the test vessels.
EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 24 and 48h exposure time. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.25 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 2.12 – 14.32 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- < 1.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No additional adverse effects or abnormal behaviour were observed in any of the test treatments.
- Mortality of control: no
- Other adverse effects control: no - Results with reference substance (positive control):
- EC50 (24 h) = 1.13 mg/L
(Experiment from 12 Feb 2009) - Validity criteria fulfilled:
- yes
Reference
DOC analysis:
The analytically determined concentrations of the test substance in the test water were 88 – 107% of the overall mean measured concentration in samples from the start of exposure and 93 - 112% of the overall mean measured concentration in samples from the end of exposure (48 h). The variability between measured replicates at each time point was generally ≤ 25%, however variability was greater in one case where the concentration was near the LOQ: 55.9% at 1.8 mg/L (48h). The test substance is a complex mixture that forms an emulsion in water. Additional samples were also sent for analysis by LC/MS as this was the only specific method available to analyze such a complex mixture at the concentrations tested. The amounts of all individual components were not determined by LC/MS analysis, but rather the mass spectra of 19 representative signals selected from a calibration mixture were evaluated. The individual components of the test substance have different characteristics and it is unlikely that all the components in the mixture will have identical behavior in solution over time. As described in the analytical report, samples of 10 mg/L (nominal) had a qualitatively similar composition according to the mass spectra. However there was a shift in signal intensity from less polar to more polar components compared to the calibration mixturewhich was greater at lower nominal concentrations. It is possible that this shift was due to binding of the less polar compounds to surfaces of the test system and thus a net shift to more polar compounds in solution. Since there was a shift in the relative composition of the mixture, the calibration procedure used for the analytical determination by LC/MS was inappropriate for a quantitative assessment. Therefore TOC values were used to calculate mean measured concentrations over the exposure period. Overall the analyses confirm that the test substance was present in the test vessels over the exposure period.
Description of key information
EC50 (48h) = 2.25 mg/L (measured) for Daphnia magna (OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.25 mg/L
Additional information
On study investigating the short-term toxicity of Fatty acids, C8-18 and C18-unsatd., reaction products with diethanolamine and propylene oxide (CAS 1000817-22-0) to aquatic invertebrates is available. The study was performed according to GLP and OECD guideline 202 using Daphnia magna as test organism (BASF 2009). After 48 hours of exposure under flow-through conditions the EC50 is determined to be 2.25 mg/L based on measured concentrations.
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