Cyclohexanethiol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1970

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was not compliant with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexanethiol

EC Number:

216-378-7

EC Name:

Cyclohexanethiol

Cas Number:

1569-69-3

Molecular formula:

C6H12S

IUPAC Name:

cyclohexanethiol

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Cyclohexyl Mercaptan
No other information reported

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 1.9 to 3.3 kg
- Housing: Standard laboratory rabbit cages
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: dorsal
- % coverage: approximately 10% body surface area
- Type of wrap if used: Plastic film secured with masking tape
- Administration: specified dose distributed over the dorsal surface by means of a syringe equipped with a blunt needle inserted between skin and wrapping

REMOVAL OF TEST SUBSTANCE
- Washing: Mild soap solution
- Time after start of exposure: 24 hours

TEST MATERIAL
- Concentration: 100%
- Constant concentration used: yes

Duration of exposure:

24 hours

Doses:

3.038, 4.556, 6.834, 10.250, 15.380 g/kg

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

Acute oral mean lethal dose (LD50) was calculated using tables contructed by Weil (based on formulae presented by Thompson and Weil and involving moving averages and interpolation as developed by Thompson).
Thompson, William R and Weil, Carrol S.: On the Construction of Tables for Moving Average Interpolation. Biometrics, March 1952
Thompson, William R.: Use of Moving Averages and Interpolation to Estimate Median-Effective Dose. Bact. Rev., Nov. 1947

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

7.82 other: g/kg

Based on:

test mat.

Mortality:

All rabbits at 15.380 g/kg died, 2 between 6 and 22 hours following dosing and 2 by Day 10.
Three rabbits at 10.25 g/kg died by Day 12
One rabbit at 6.834 g/kg died between 6 and 22 hours following dosing
One rabbit at 4.556 g/kg died by Day 5

Clinical signs:

other: Generalised weakness, edema, erythma and drying of the skin at the application sites was noted in all groups.

Gross pathology:

Ischemic lungs, hyperemic liver and necrosis of the skin at the application site were noted in all groups except 3.038 g/kg.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Acute dermal LD50 in rabbits was 7.82 g/kg +/- 1.66 g/kg