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EC number: 412-720-6 | CAS number: 156653-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 June 1993 to 04 August 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to EU & OECD test guidance in compliance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 79/831/EEC
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- See below
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sample concentrations: 0, 560, 1000 mg/l
In order to determine the substance concentration, water samples were taken at study start and study end. The concentration value of the 1000 mg/l-group decreased from 89.5 % (895 mg/l) of the nominal
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was weighed directly into the test chambers, mixed with water for dilution and homogenized with the Ultra-Turrax and ultrasonic bath. The test batches were present as yellow solutions.
No separation or formation of deposits was observed with any of the concentrations tested. In order to determine the substance concentration, water samples were taken at study start and study end. The concentration value of the 1000 mg/l-group decreased from 89.5 % (895 mg/1) of the nominal concentration at study start to 77.5 % (775 mg/1) after 48 hours.
All concentration-values of the 560 mg/l-group were in the range of ± 20 % of the nominal concentration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna - Clone 5 (IRChA, France)
Origin: Stockculture of the Department of Toxicology (Hoechst AG)
Breeding conditions: 100 ml beakers (height 90 mm, diameter 50 mm), filled with 70 ml test water (treated fresh water, for composition see Appendix page 18) served as culture vessels. One daphnid was used per culture vessel. The animals were used for breeding up to an age of 42 days. The reproduction rate and the state of health of the animals were monitored daily apart from weekends. The animals were fed three times a week with monocellular green algae (Scenedesmus subspicatus).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation.
Test conditions
- Hardness:
- Recorded by not reported
- Test temperature:
- 20.4 - 21.5C
- pH:
- 7.5 - 8.6
- Dissolved oxygen:
- 8.5 - 8.8mg/l
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0, 560, 1000 mg/l (nominal)
- Details on test conditions:
- The study was conducted in a static system. The test chambers were 250 ml beakers (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 ml test water. The room temperature was about 21⁰C. The room was illuminated by fluorescent tubes in a day/night cycle, with each period lasting 12 hours.
After the test concentrations had been prepared and water parameters recorded, 10 daphnids were used in each test chamber. The daphnids received no feed for the entire study period. Inspection of the daphnids took place after 24 and 48 hours and involved recording the immobility. Immobile daphnids were removed from the chambers. Daphnids were considered immobile when there was a 1 ack of movement 15 sec. after touching the test chamber. The water parameters were measured after adding the substance and at the end of the test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In this 48-hour acute toxicity study of Reaktiv-Gelb F66 923 FW in daphnia magna no immobility occurred in the 560 and 1000 mg/l group. The mean of the analytical determined concentration-values in the 1000 mg/1 group was 835.0 mg/l.
The LC50 after 96 hours therefore exceeds 1000 mg/l (nominal concentration) or 835.0 mg/l (mean of the analytical determined concentration), respectively. - Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- No data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 48-hour acute toxicity study of Reaktiv-Gelb F66 923 FW in daphnia magna no immobility occurred in the 560 and 1000 mg/l group. The mean of the analytical determined concentration-values in the 1000 mg/l group was 835.0 mg/l.
The LC50 after 96 hours therefore exceeds 1000 mg/1 (nominal concentration) or 835.0 mg/1 (mean of the analytical determined concentration), respectively.
The substance is not harmful to Daphnia. - Executive summary:
The acute toxicity of Reaktiv-Gelb F-66 923 FW in Daphnia magna (waterflea) was determined in accordancewith the requirements of EEC-Guideline, EEC Directive 79/831 Annex V, Methods for the Determination of Ecotoxicity 5.1.2 Acute Toxicity for Daphnia as from Feb. 1999 and the OECD-Guideline, OECD-Guideline for testing of chemicals, Section 2 - Effects on Biotic Systems Test Guideline 202 Daphnia sp., 14 -day Reproduction Test (including an Acute Immobilisation Test) Adopted: April 4, 1984. Study conducted in complince with GLP.
When testing the immobility effect of Reaktiv-Gelb F-66 923 FW in Daphnia magna (water flea) over a total of 48 hours according to the method described under section 4, no lethality occurred at 1000 mg/l.The concentrations tested were 560, 1000 mg/l and a negative control (0 mg/l).
All tested concentrations were present as yellow solutions.
All test batches were homogeneous. No particulate matter was observed.
The mean of the analytical determined concentration-values in the 1000 mg/l group was 835.0 mg/l.
The EC50 after 48 hours therefore exceeds 1000 mg/l (nominal concentration) or 835.0 mg/l (mean of the analytical determined concentration), respectively.
The concentration value of the 1000 mg/l-group decreased from 89.5 % of the nominal concentration at study start to 77.5% after 48 hours. All concentration-values of the 560 mg/l-group were in the range of + 20 % of the nominal concentration.
The test substance is not considered to be harmful to Daphnia.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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