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EC number: 205-206-6 | CAS number: 135-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: According to OECD 301 B with deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- 56 day study
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-2'-methoxy-2-naphthanilide
- EC Number:
- 205-206-6
- EC Name:
- 3-hydroxy-2'-methoxy-2-naphthanilide
- Cas Number:
- 135-62-6
- Molecular formula:
- C18H15NO3
- IUPAC Name:
- 3-hydroxy-N-(2-methoxyphenyl)naphthalene-2-carboxamide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Frankfurt-Sindlingen
- Pretreatment: according to guideline, the supernatant was decanted and maintained in an aerobic condition by aeration with for 3 days- 10 mL/L were used to initiate inoculation - Duration of test (contact time):
- 56 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20.33 mg/L
- Based on:
- other: nominal concentration of test material
- Initial conc.:
- 20.53 mg/L
- Based on:
- other: nominal concentration of test material
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ± 2°C; deviation on days 55 and 56 (16°C)
TEST SYSTEM
- Culturing apparatus: 5000 mL glass flasks
- Number of culture flasks/concentration: 2 for the reference item, 2 for the control, 2 for the test item
- Details of trap for CO2 and volatile organics if used: CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 vessels, each containing 100 mL of a 0.0125 mol/L Ba(OH)2 solution.
SAMPLING
- Sampling frequency: Backtitration of the residual Ba(OH)2 with 0.05 N HCL was carried out three times a week during the first ten days and thereafter twice weekly.
- Sampling method: 25 mL respectively were taken to determine the residual Ba(OH)2 by titration
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg/L
Results and discussion
- Preliminary study:
- Not performed
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 47.8
- Sampling time:
- 56 d
- Remarks on result:
- other: mean of duplicates
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 15
- Sampling time:
- 56 d
- Remarks on result:
- other: sample A
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 80.5
- Sampling time:
- 56 d
- Remarks on result:
- other: sample B
- Details on results:
- The total amount of CO2 produced in 56 days was analysed by titration in 9 measurements respectively.
The test item reached the 10 % level (beginning of biodegradation) after a long lasting adaptation phase of 21 days.
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days. In one of the two vessels the biodegradation was > 60 % after 56 days. Therefore the substance has to be regarded as potentially biodegradable.
BOD5 / COD results
- Results with reference substance:
- The course of the degradation was rapid and the functional control reached the pass level of 60 % within 7days. The validity criterion degradation >/= 60 % after 14 d is fulfilled.
Any other information on results incl. tables
Day of study |
CO2-formation [mg C/L] |
||||||
100 % A = 14.99; 100 % B = 15.13 |
|
100 % = 11.61 |
|||||
|
A |
B |
A [%] |
B [%] |
Mean [%] |
Reference |
[%] |
3 |
0.15 |
0.39 |
1.0 |
2.6 |
1.8 |
3.75 |
32.3 |
7 |
0.25 |
0.59 |
1.6 |
3.9 |
2.8 |
7.59 |
65.3 |
14 |
0.31 |
0.73 |
2.1 |
4.8 |
3.4 |
9.64 |
83.0 |
21 |
0.46 |
2.87 |
3.0 |
18.9 |
11.0 |
10.47 |
90.1 |
27 |
0.59 |
4.33 |
3.9 |
28.6 |
16.2 |
10.66 |
91.8 |
34 |
0.67 |
6.85 |
4.4 |
45.2 |
24.8 |
|
|
41 |
0.70 |
8.72 |
4.6 |
57.6 |
31.1 |
|
|
55 |
1.14 |
10.40 |
7.6 |
68.7 |
38.1 |
|
|
56 |
2.26 |
12.19 |
15.0 |
80.5 |
47.8 |
|
|
A,B: Testsubstance
Applicant's summary and conclusion
- Interpretation of results:
- inherently biodegradable, not fulfilling specific criteria
- Conclusions:
- The test item must be regarded as not readily biodegradable in the 10 d-window and after 28 days.
- Executive summary:
The ready biodegradability of the test item was determined with a non adapted activated sludge over a test period of 56 days in the Modified Sturm Test. The study was conducted according to OECD 301 B. The test item was tested at a concentration of 20 mg/L in duplicates, corresponding to a carbon content (DOC) of 15 mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 56. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation rate was calculated for each titration time.
To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 7 days and came to a maximum of 91.8 % after 27 days.
The test item reached the 10 % level (beginning of biodegradation) after a long lasting adaptation phase of 21 days. Therefore the test duration was prolonged to 56 days. The degradation rate after 56 days was 15% and 80.5:%
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days. In one of the two vessels the biodegradation was > 60 % after 56 days. Therefore the substance has to be regarded as potentially biodegradable.
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