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EC number: 627-083-1 | CAS number: 244235-47-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Dec 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: A fresh sample of activated sludge trom the aeration basin of a municipal sewage treatment plant (Moers, Germany) was collected one day before test start
- Preparation of inoculum for exposure: The activated sludge was filtered by means of a water jet pump through a filter paper. The sludge was washed twice with mineral medium. Afterwards, 4 g/L activated sludge was suspended in mineral medium, 50 mL synthetic sewage was added and the whole mixture was stirred and aerated obernightat 20 °C.
- Initial biomass concentration: 3 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- not specified
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.27 - 8.16
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1L beakers (incubation period) + 300 mL Karlsruher flasks for O2 measurement
- Aeration: The incubation vessels were aerated with compressed synthetic air (0.5 - 1.0 L/min) under permanent stirring with a magnetic stirrer.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: The pH was measured at test start and after 3h contact time.
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: O2 consumption was measured after a contact time of 3 hours for 10 minutes using an oxygen electrode. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 260.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: yes, since undissolved test substance was not removed from the test solution
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP study according to OECD guideline 209, a 3h-EC50 value of the test substance was > 1000 mg/L, while the respective 3h-EC10 value is determined to be 260.1 mg/L.
- Executive summary:
The toxicity of the test substance to microorganisms was evaluated in a study according to OECD guideline 209 under GLP conditions.
In a static study domestic activated sludge was exposed for 3h. The test material was applied directly to the test vessels.
Analytical monitoring was not performed. All validity criteria were fulfilled. The 3h-EC50 is determined to be > 1000 mg/L, while the respective 3h-EC10 value is dtermined to be 260.1 mg/L based on the nominal concentration.
Reference
Description of key information
EC50 (3h) > 1000 mg/L, EC10 (3h) = 260.1 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 260.1 mg/L
Additional information
One study investigating the toxicity of the test substance to aquatic microorganisms is available. The study was performed according to GLP and OECD guideline 209 (Wehrhahn 2010). In a static study domestic activated sludge was exposed for 3h. The test material was applied directly to the test vessels. Analytical monitoring was not performed. All validity criteria were fulfilled. The 3h-EC50 is determined to be > 1000 mg/L, while the respective 3h-EC10 value is determined to be 260.1 mg/L based on the nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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