2-chlorobenzothiazole

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorobenzothiazole

EC Number:

210-415-0

EC Name:

2-chlorobenzothiazole

Cas Number:

615-20-3

Molecular formula:

C7H4ClNS

IUPAC Name:

2-chloro-1,3-benzothiazole

Details on test material:

purity > 99.4 % w/w

Method

Target gene:

His synthesis

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Test concentrations with justification for top dose:

First series +/-S9-mix: 0, 8, 40, 200, 1000, 5000 µg/plate
Second series with vehicle ethylene glycol dimethylether (EDGE) +/-S9-mix: 0, 15, 30, 60, 120, 240, 480, 960 µg/plate

Vehicle / solvent:

ethylene glycol dimethylether (EDGE)

Controls

Controls 1

Positive controls:

yes

Positive control substance:

sodium azide

Controls 2

Positive controls:

yes

Positive control substance:

other: nitrofurantoin, 4-nitro-1,2-phenylene-diamine, 2-aminoanthracene

Details on test system and experimental conditions:

with and without metabolic activation (S9 mix)
First series +/-S9-mix: 0, 8, 40, 200, 1000, 5000 µg/plate
Second series with vehicle ethylene glycol dimethylether (EDGE) +/-S9-mix: 0, 15, 30, 60, 120, 240, 480, 960 µg/plate

Evaluation criteria:

Functionality of positive controls

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

5000 µg/plate without vehicle, 120 µg/plate with vehicle

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

2-Chlorobenzothiazole was not mutagenic in a test according to OECD Guideline 471 "Ames test" with 4 Salmonella typhimurium strains with and without metabolic activation

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