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EC number: 226-724-9 | CAS number: 5459-04-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well performed OECD and GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Principles of method if other than guideline:
- A limit test at a dose level of 5000 mg/kg was carried out.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ethylene diacetoacetate
- EC Number:
- 226-724-9
- EC Name:
- Ethylene diacetoacetate
- Cas Number:
- 5459-04-1
- Molecular formula:
- C10H14O6
- IUPAC Name:
- ethane-1,2-diyl bis(3-oxobutanoate)
- Details on test material:
- - Name of test material (as cited in study report): Acetessigsäure-glykolester
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund
- Age at study initiation: males: about 7 weeks; females: 9 weeks
- Weight at study initiation: average: males 194 Grams; females 189 Grams
- Fasting period before study: 16 hours
- Housing: air conditioned rooms; macrolone cages with groups of 5 animals
- Diet (e.g. ad libitum): rat diet Altromin 1324 ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimatisation period: at leats 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +-3 °C
- Humidity (%): 50+-20 %
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 50% (w/v) - Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5 males
5 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: daily
Results and discussion
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 1 of 5 females
0 of 5 males - Clinical signs:
- other: squatting posture, flanks pinched in, irregular respiration, stilted gait, lateral position, prone position, decreased spontaneous activity, coat bristling, increased respiratory rate
Any other information on results incl. tables
No abnormal finding in animals sacrificed at the end of the observation period. One female found dead at day 2 could not be examinined due to cannibalism.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- The LD50 for acute orale toxicity in rats is > 5000 mg/kg bw.
- Executive summary:
Acetessigsäure-glykolester was tested for acute oral toxicity following the protocol of OECD 401 (limit test). A single dose of 5000 mg/kg bw was tested by administering a 50% (W/V) emulsion in water once per oral gavage to 5 male and 5 female Wistar rats. Median body weights were 194 g (m) and 189 g (f). 16 h before until 2 h after administration food was withdrawn. The post administrational observation period was 14 d. During this time rats were fed with standard diet and tap water.
During the observation period lethality was 1 (f) out of 10 animals (m and f).These findings demonstrate that oral LD50 exceeds 5000 mg/kg bw.
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