Manganese, [[2,2',2''-[nitrilotris[2,1-ethanediyl(nitrilo-.kappa.N)methylidyne]]tris[phenolato-.kappa.O]](3-)]-, (OC-6-22)-

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-10-28 to 2003-01-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline study, GLP. No test could be performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

adopted January 2001

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

August 2001

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

479-540-8

EC Name:

-

Cas Number:

61007-89-4

Molecular formula:

C27 H27 Mn N4 O3

IUPAC Name:

manganese(3+) 2-[({2-[bis(2-{[(2-oxidophenyl)methylidene]amino}ethyl)amino]ethyl}imino)methyl]benzen-1-olate

Radiolabelling:

no

Study design

Test temperature:

25 °C

HPLC method

Details on study design: HPLC method:

TEST ITEM SOLUTIONS
- Test substance was dissolved in DMF (20 ml) to prepare a stock solution of 1600 µg/mL. This solution was injected through the HPLC.
- Tests to determine the adsorption coefficient (Koc) were performed with methanol and with acetonitrile as organic solvents. These solvents were not sufficient to elute the test item from the column. During the runs, no test item peak was detected.

EQUIPMENT
- Apparatus: Varian autosampler 9095; Varian pump 230; Jones column oven 7990
- Type, material and dimension of analytical column: LIChrospher 100 CN, part, size 5 pm, 250 mm x 4 mm

REFERENCE SUBSTANCES
- No reference items were used

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µL


EVALUATION
- Calculation of capacity factors k': The capacity factor (k') was calculated for each reference item using the retention times.
- Determination of the log Koc value: The log Koc was calculated using a regression curve (log k' vs. log Koc)

Results and discussion

Results: HPLC method

Details on results (HPLC method):

The test item is not stable in aqueous solution, hydrolysis occurs immediately by contact with water . During the test no suitable solvent was found to elute the test item from the HPLC column. Acetonitrile and methanol were testes and were not sufficient as no test item was eluted from the column.
Therefore, the adsorption coefficient (Koc) of the test substance could not be determined using the HPLC method.