Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-485-4 | CAS number: 2162-73-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-03-21 - 1989-05-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- Slight modifications from the guideline (e.g. precise description and scoring of corneal defects, additional examination of aqueous humour) do not affect the validity of the outcome of this study.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,4,6-triisopropyl-m-phenylene diisocyanate
- EC Number:
- 218-485-4
- EC Name:
- 2,4,6-triisopropyl-m-phenylene diisocyanate
- Cas Number:
- 2162-73-4
- Molecular formula:
- C17H22N2O2
- IUPAC Name:
- 2,4-diisocyanato-1,3,5-tris(propan-2-yl)benzene
- Test material form:
- liquid
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- healthy adult albino rabbits, females were nulliparous and not pregnant.
- Source: breeder Interfauna U.K. Ltd., Wyton, Huntingdon, England
- Age at study initiation: adult
- Weight at study initiation: 3.0 - 4.0 kg (weighed directly before application of the test compound)
- Housing: under standardised conventional conditions in individual stainless steel cages. Excrement trays beneath the cages contained low-dust wood shavings (type S 8/15, Ssniff Spezialdiäten GmbH, Soest). Shavings were regularly spot-checked for contaminants at the instance of the Department of Laboratory Animal Services.
All the animals in this study were kept in one room. For capacity reasons, rabbits treated with other test compounds were also housed in the same room. Adequate separation, clear cage and individual marks, and appropriate organisation of working procedures ensured that the test animals were not mixed up.
- Cleaning, disinfection; pest control: room surfaces are cleaned at least once per month, and the room floor once per week, and disinfected with Zephirol®-10% (1% in water). In so doing contamination of the diet and contact with the animals is excluded. No pest control was performed in the animal rooms. The drinking apparatus were cleaned once a week.
- Diet (e.g. ad libitum): standard diet "Ssniff K 4" (Ssniff Spezialdiäten GmbH, Soest), approx. 100 - 120 g per animal and day; feeding once per day in the morning.
- Water (e.g. ad libitum): tap water was available ad libitum (between 7 and 19 hrs. by automatic watering apparatus)
- Acclimation period: at least 14 days in the quarantine station of the Department of Laboratory Animal Services before treatment and monitored for diseases (clinical). shortly after arrival collective faeces specimens were examined for Coccidia oocysts.
Vaccination or treatment with antibiotics was performed neither prior to receipt of the animals nor during the acclimatization or study period.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 3 °C
- Humidity (%): approx. 50 %
- Air changes (per hr): approx. 10 times per hour
- Photoperiod (hrs dark / hrs light): 12 hours, artificial illumination from 6 to 18 hrs CET
- Intensity of illumination: approx. 27 Watt/m2 floor area
RANDOMIZATION
The individual rabbits were randomly assigned to the respective treatment groups. Randomisation was performed by means of a random number generator with varying starting conditions, using an Apple 2e.
For reasons of animal welfare, rabbits from former studies that tolerated treatment without signs are also used. In these animals the opposite skin area/eye which served as control in the preceding study is treated in the following study to prevent interactions of different test compounds.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 100 µl
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- 72 hours (If irritation was observed, animals were monitored for not more than 21 days after application)
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): with physiological NaCl solution
- Time after start of exposure: 24 hours
SCORING SYSTEM: cornea, fluorescein staining (epithelial defects), iris, aqueous flare, conjunctivae and discharge
TOOL USED TO ASSESS SCORE: hand-slit lamp
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- H7
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- H7
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- H7
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Remarks:
- H25
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Remarks:
- H25
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Remarks:
- H25
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Remarks:
- H26
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Remarks:
- H26
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Remarks:
- H26
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- H7
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Remarks:
- H25
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Remarks:
- H26
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- No irritation of the eye was noted after the end of the observation period.
- Other effects:
- No other findings stated
Any other information on results incl. tables
Table 1: Test for irritant effect on the eye (expos . 24 hrs)
Animal no. (body weight) | tissue | signs | DRAIZE grades | Irritation index | ||||||
1 h | 24 h | 48 h | 72 h | 7 d | 14 d | 21 d | ||||
H7(3.4 kg) | cornea | o | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 |
a | 0 | 0 | 0 | 0 | 0 | - | - | |||
fluorescein | i | - | 0 | - | - | - | - | - | ||
a | - | 0 | - | - | - | - | - | |||
iris / conjuncitvae / conjunctivae | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
r | 1 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
s | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
aqueous humour discharge | 0 | 0 | 0 | 0 | 0 | - | - | |||
1 | 0 | 0 | 0 | 0 | - | - | ||||
H25 (3.1 kg) | cornea | o | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 |
a | 0 | 0 | 0 | 0 | 0 | - | - | |||
fluorescein | i | - | 0 | - | - | - | - | - | ||
a | - | 0 | - | - | - | - | - | |||
iris / conjunctivae / conjunctivae | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
r | 1 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
s | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
aqueous humour discharge | 0 | 0 | 0 | 0 | 0 | - | - | |||
1 | 0 | 0 | 0 | 0 | - | - | ||||
H26 (3.2 kg) | cornea | o | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 |
a | 0 | 0 | 0 | 0 | 0 | - | - | |||
fluorescein | i | - | 0 | - | - | - | - | - | ||
a | - | 0 | - | - | - | - | - | |||
iris / conjunctivae / conjunctivae | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
r | 1 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
s | 0 | 0 | 0 | 0 | 0 | - | - | 0.0 | ||
aqueous humour discharge | 0 | 0 | 0 | 0 | 0 | - | - | |||
1 | 1 | 1 | 1 | 0 | - | - |
o = opacity
a = area
i = intensity
r = redness
s = swelling
- = not examined
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The study was performed according to the OECD TG 405 with only minor deviations and according to the good laboratory practice principles, it is considered to be of the highest quality (reliability Klimisch 1). The criteria of validity of the test system are fulfilled. The test material did not induce any irritation or corrosion on rabbit eyes. The test material was considered to be not irritating under the conditions of the test (exposure duration 24 hours).
- Executive summary:
Triisopropylbenzoldiisocyanat was investigated for its potential to cause irritation in the rabbit eye according to OECD TG 405 (Märtins, 1989). 100 µL of pure substance were instilled into the conjunctival sac, the untreated eye served as control. Interpretation of results was based on the nature, intensity, and reversibility of the responses observed. Under the conditions of this experiment the test compound was found to cause no irritation when applied to the rabbit eye mucosa. No corrosion effect was observed at each of the measuring intervals.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.