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EC number: 254-100-6 | CAS number: 38720-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 23 SEP 1970 to 23 Oct 1970
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Basic data given: comparable to guidelines (limitations: e.g. no clinical biochemistry, no detailed clinical observations, no functional observations, only 4-5 days post observation period, no reporting of individual/mean data, especially on food consumption and body weight development)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
- Principles of method if other than guideline:
- subacute toxicity test
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 254-100-6
- EC Name:
- Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Cas Number:
- 38720-66-0
- Molecular formula:
- C20H10Cl2N2O2
- IUPAC Name:
- 1,8-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 1,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 2,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: in house breeding; SPF
- Weight at study initiation: males: mean 147 g (122 g - 172 g); females: mean 110 g (90 g - 126 g)
- Fasting period before study: no
- Housing: 5 animals/sex/cage
- Diet: Standard diet Altromin R (Altromin GmbH, Lage, Germany), ad libitum
- Water: tap water, ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-25
- Humidity (%): 35-60
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- DIET PREPARATION
- Rate of preparation of diet (frequency): once at the beginning of the study
- Mixing appropriate amounts with standard diet using a "Lödige-Präzisions-Minuten-Mischer Modell M 20 E"
- Preparation of pellets using a "Templewood-Mischfutterpresse"
- Storage temperature of food: no data - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 30 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 0.2, 1.0 and 5% in diet
Basis:
nominal in diet
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, plain diet
- Details on study design:
- - Dose selection rationale: LD50 after single oral application > 15000 mg/kg bw; pretest (10 days feeding study) with rats receiving feed with 5%, 1% and 0.2% of the test item
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: daily
DETAILED CLINICAL OBSERVATIONS: No
BODY WEIGHT: Yes
- Time schedule for examinations: weekly
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study):
- Food consumption for each animal determined: YES
- mean daily diet consumption calculated as g food/kg body weight/day: No data
- Compound intake calculated as time-weighted averages from the consumption and body weight gain data: No data
FOOD EFFICIENCY:
- Body weight gain in kg/food consumption in kg per unit time X 100 calculated as time-weighted averages from the consumption and body weight gain data: No data
WATER CONSUMPTION: No
OPHTHALMOSCOPIC EXAMINATION: No
HAEMATOLOGY: Yes
- Time schedule for collection of blood: before the beginning and at the end of the experiment
- Anaesthetic used for blood collection: No data
- Animals fasted: No data
- How many animals: from each animal
- Parameters examined: haemoglobin concentration, erythrocyte count, leucocyte count, differential blood count, appearance of Heinz bodies
CLINICAL CHEMISTRY: No
URINALYSIS: Yes
- Time schedule for collection of urine: before the beginning and at the end of the experiment
- Metabolism cages used for collection of urine: No data
- Animals fasted: No data
- Parameters examined: appearance, colour, protein, glucose, bilirubin, specific weight (urine collected from 5 animals), sediment
NEUROBEHAVIOURAL EXAMINATION: No - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes (heart, lung, liver, spleen, adrenals, kidney) - Other examinations:
- - heart, lung, liver, spleen, adrenals, kidney
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- no effects observed
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not examined
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- no effects observed
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY
- no effects
BODY WEIGHT AND WEIGHT GAIN
- no differences between treated animals and controls
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study)
-no effects
HAEMATOLOGY
- no pathological findings
URINALYSIS
- no test item related effects
- at the end of the study male rats excreted protein in the urine which was judged to be normal in adult male rats
ORGAN WEIGHTS
- no effects
GROSS PATHOLOGY
- no effects
HISTOPATHOLOGY: NON-NEOPLASTIC
- no effects
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- >= 50 000 mg/kg diet
- Sex:
- male/female
- Basis for effect level:
- other: no toxic effects observed, 5% in diet correspond to 2000 and 2500 mg/kg bw/day for males and females, respectively, calculated according to ECHA Guidance on information requirements R.8
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
Compared to the control group, no alterations in the general behaviour, food uptake and body weight gain of the treated animals were observed. The examination of blood and urine revealed no toxic responses (proteinuria in all males was regarded as typical for male rats).
Organ weights of the animals were within the normal range. Macroscopic and microscopic examination of heart, lung, liver, spleen, adrenals and kidneys revealed no pathological findings.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this study the test item did not produce toxic responses in the treated animals.
- Executive summary:
Wistar rats (10 per sex and group) were fed diets with the test item in concentrations of 0, 0.2, 1 and 5% for 30 consecutive days. There were no toxic responses compared to the controls with respect to general behaviour, food uptake, body weight gain. The examination of blood and urine revealed no toxic responses (proteinuria in all males was regarded as typical for male rats). Organ weights of the animals were within the normal range. Macroscopic and microscopic examination of heart, lung, liver, spleen, adrenals and kidneys revealed no pathological findings. The NOAEL was 5% test item in the diet (corresponding to 2000 and 2500 mg/kg bw/day for males and females, respectively).
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