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EC number: 298-680-9 | CAS number: 93820-97-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Apr - 24 Apr 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions (The highest test concentration was slightly turbid which probably has affected the outcome of this study).
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
- Reference Type:
- secondary source
- Title:
- National Industrial Chemicals Notification and Assessment Scheme - Full Public Report - [trade name]
- Author:
- Australian Government - Department of Health and Ageing NICNAS
- Year:
- 1 999
- Bibliographic source:
- File No.: NA/655
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-octyldodecyl isooctadecanoate
- EC Number:
- 298-361-4
- EC Name:
- 2-octyldodecyl isooctadecanoate
- Cas Number:
- 93803-87-3
- Molecular formula:
- C38H76O2
- IUPAC Name:
- 2-octyldodecyl isooctadecanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Two solutions were prepared at nominal concentrations of 10 and 100 mL respectively in order to achieve maximum exposure concentrations. These solutions were stirred for approx. 21 h, stabilized for 4 h and separated using a separation funnel afterwards. After preparation, volumes of 50 mL were added to each replicate of the respective test concentration.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
- Evidence of undissolved material: The separated solutions were slightly turbid.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source: Laboratory culture
- Method of cultivation: Algae stock cultures were started by inoculating growth medium with algal cells from pure culture on agar. The suspensions were continuously aerated and exposed to light (4000-9000 lux) in a climate room at a temperature of 23 ± 2 °C.
ACCLIMATION
- Acclimation period: 4 d before the start of the test, cells from the algal stock culture were inoculated in a culture medium at a cell density of 2x10^4 cells/mL.
- Culturing media and conditions (same as test or not): same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 20 - 22 °C
- pH:
- 8.3 - 8.5
- Nominal and measured concentrations:
- nominal: 0, 10 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size: all-glass, 100 mL
- Aeration: continuously shaking
- Initial cells density: 1x10^4 cells/mL
- Control end cells density: 47.5 x 10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Other: One vessel of each test concentration without algae.
GROWTH MEDIUM
- Standard medium used: yes (according to ISO guideline)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water purified by reverse osmosis and then passed over activated carbon and ion-exchange cartridges.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuously illuminated
- Light intensity and quality: TLD-lamps of 18 Watt yielding 6000 - 6500 lux.
EFFECT PARAMETERS MEASURED: growth rate (every 24 h)
- Determination of cell concentrations: At the beginning of the test, cells were counted by microscope using a counting chamber. Thereafter, cell densities were determined by spectrophotometric measurement of samples at 720 nm using a Lambda Spectrophotometer (Perkin-Elmer, Illinois, USA), with a cuvette of 5 cm path-length. Algal medium was used as blank and the extra replicates as background for the treated solutions.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10x - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Test solution at nominal 100 mg/L was slightly turbid. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 1.7 mg/L based on growth rate reduction (95% CL: 1.1 - 2.8 mg/L)
Any other information on results incl. tables
Cell growth was inhibited significantly (31.2%) at nominal 100 mg/L, while cell growth was not affected at nominal 10 mg/L. This significant effect is probably related to the turbid test solution which adsorbed a fraction of light needed for algal cell growth. Since both test concentrations are above water solubility it can be concluded that both the EC 50 and the NOEC exceed the maximum possible water solubility of the test substance.
Table 1: Percentage reduction of growth rate at different time intervals.
Concentration of test substance: |
Cell growth inhibition (0-72 h): |
Growth rate [cells/mL/h-1] |
||
Area (mean) |
Inhibition [%] |
mean µ1 |
Reduction [%] |
|
blank |
894.41 |
-- |
0.05360 |
-- |
10 mg/L |
855.12 |
4.4 |
0.05204 |
2.9 |
100 mg/L |
615.50 |
31.2 |
0.05140 |
4.1 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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