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EC number: 229-494-8 | CAS number: 6574-99-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- december - february 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Qualifier:
- according to guideline
- Guideline:
- other: NEN-EN-ISO 10712 (1996)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - incubation temperature: 23 +/- 1 °C
- incubation time (preliminary suspension): 6 +/- 1 hours
- incubation time (exposure): 16 +/- 2 hours
- Stock solution I: 10.0 g NaNO3, 2.4 g K2HPO4, 1.2 g KH2PO4, 1.0 g yeast extract, dissolved in 500 mL Milli-Q water
- Stock solution II: 10.0 g NaNO3, 2.4 g K2HPO4, 1.2 g KH2PO4, dissolved in 500 mL Milli-Q water
- Stock solution III: 40.0 g D(+)-glucose monohydrate dissolved in 500 mL Milli-Q water
- Stock solution IV: 0.01 g Fe(III)citrate, 4.0 g MgSO4x7H2O, dissolved in 1L Milli-Q water
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
- Remarks on exposure duration:
- in an incubator shaker in the dark
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 17 h
- Dose descriptor:
- EC10
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC10 of 3,4-Dichlorobenzonitrile was calculated at 56 mg/l; the EC50 was ca. 79 mg/l.
- Executive summary:
3,4 -Dichlorobenzonitrile was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996).
Suspensions of Pseudomonas putida were exposed to concentrations ranging from 3.1 to 100 mg 3,4-Dichlorobenzonitrile per liter. After incubation for 17 h the extinction values of the bacterial cell suspensions were measured. Significant (significant: >10%) toxicity was observed at 100 mg/l only.
Based on the results of the test presently performed, the toxicity threshold value (EC10) of 3,4-Dichlorobenzonitrile was ca. 56 mg/L. The "Bewertungszahl" (Assessment figure or value for bacterial toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1989). The EC50 was ca. 79 mg/L.
The EC50 value for the reference substance, 3,5 -Dichlorphenol, tested during the same experiment was 22 mg/L. Since this value was within the accepted range of 10 -30 mg/L for the EC50, and the multiplication factor of the bacteria within the test substance was over 60, it was concluded that the test conditions were optimal and the resluts obtained were valid.
Reference
After incubation extinction values were measured and the cell multiplication inhibition for each test concentration was determined (table 3). Severe toxicity was observed in two of the three vessels at 100 mg/L only. No turbidity was observed in the turbidity controls.
Since it was not clear which of the three vessels at 100 mg/L gave the correct value a second test was performed in which only the three highest concentrations, being 100, 50 and 25 mg/L, were tested. The method and performance of this test were the same as in the first test. The results indicated clear toxicity at 100 mg/L. Therefore, the value I at 100 mg/L in the first test was considered to be an outlying value and excluded from further calculations.
Since no inhibition was seen at 50 mg/L and 87 % inhibition at 100 mg/L, the EC50 -value for growth inhibition was calculated to be approx. 79 mg/L, whereas the EC10 value was approx. 56 mgL.
Table 3: Extinction values of the inoculated dilution series I, II, III and the non-inoculated dilution series t (turbidity control).
concentration 3,4-Dichlorobenzonitrile [mg/L] |
extinction in FNU 436 nm |
inhibition % |
|||||
series |
|
|
mean |
t |
corrected mean |
||
I |
II |
III |
I, II, III |
||||
3.1 |
1.848 |
1.770 |
1.656 |
1.758 |
0.009 |
1.749 |
2.7 |
6.3 |
1.770 |
1.679 |
1.790 |
1.746 |
0.008 |
1.738 |
3.3 |
12.5 |
1.694 |
1.771 |
1.712 |
1.726 |
0.007 |
1.719 |
4.4 |
25 |
1.760 |
1.776 |
1.751 |
1.762 |
0.007 |
1.755 |
2.3 |
50 |
1.890 |
1.927 |
1.763 |
1.860 |
0.007 |
1.853 |
-3.2 |
100 |
1.5921) |
0.278 |
0.227 |
0.253 |
0.007 |
0.246 |
86.9 |
1) outlying value, excluded from further calculations.
Acceptability of the test:
The EC50 value of 3,5 -Dichlorophenol (reference substance) determined from Pseudomonas putida as calculated by linear regression (Y = 3.1209 X - 18.9803) was 22 mg/L.
The inoculum used in the control (4 FNU) was multiplied by a factor 150 within the test period.
With respect to the accepted range of 10 -30 mg/L for the EC50 of the reference substance and the multiplication factor of the bacteria within the test being over 60, it was concluded that the test conditions were optimal and the results obtained were valid.
Description of key information
The toxicity threshold value (EC10) and the EC50 value of 3,4 -Dichlorobenzonitrile was determined to be
ca. 56 mg/L and 79 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 79 mg/L
- EC10 or NOEC for microorganisms:
- 56 mg/L
Additional information
3,4 -Dichlorobenzonitrile was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996).
Suspensions of Pseudomonas putida were exposed to concentrations ranging from 3.1 to 100 mg 3,4-Dichlorobenzonitrile per liter. After incubation for 17 h the extinction values of the bacterial cell suspensions were measured. Significant (significant: >10%) toxicity was observed at 100 mg/l only.
Based on the results of the test presently performed, the toxicity threshold value (EC10) of 3,4-Dichlorobenzonitrile was ca. 56 mg/L. The "Bewertungszahl" (Assessment figure or value for bacterial toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1989). The EC50 was ca. 79 mg/L.
The EC50 value for the reference substance, 3,5 -Dichlorphenol, tested during the same experiment was 22 mg/L. Since this value was within the accepted range of 10 -30 mg/L for the EC50, and the multiplication factor of the bacteria within the test substance was over 60, it was concluded that the test conditions were optimal and the results obtained were valid.
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