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EC number: 413-920-6 | CAS number: 88949-33-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and OECD testing guideline compliant study with well characterized test material
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted May 26, 1983
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Details on test material:
- - Substance type: Organic
- Physical state: Solid
- Lot/batch No.: Z-2281/1,2,4,5
- Expiration date of the lot/batch: April 01, 1997
- Storage condition of test material: at room temperature in the dark
Constituent 1
Method
- Target gene:
- His: Salmonella
Trp: E. Coli
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium strains: TA100, TA98, TA1535, TA1537; E. coli : WP2uvrA and WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 mix
- Test concentrations with justification for top dose:
- 10; 100; 333.3; 1000; and 5000 µg/plate
- Vehicle / solvent:
- Dimethyl sulfoxide
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see: "Details on test system and conditions"
- Details on test system and experimental conditions:
- POSITIVE CONTROLS:
Without metabolic activation (-S9-mix):
TA1535, TA 100: sodium azide (SA), 10 µg
TA1537, TA 98: 4-nitro-o-phenylene-diamine, 4-NOPD 50 µg
WP2uvrA, WP2 : methylmethanesulfonate (MMS), 10 µl
With metabolic activation (+S9-mix):
TA1537, : 2-aminoanthracene (2AA), 2.5 µg
TA1535, TA 1537, TA98 and TA100: 2-aminoanthracene (2AA), 2 µg
WP2uvrA and WP2: 2-aminoanthracene (2AA), 2 µg
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Incubation period: 48 hours. After this period revertant colonies (histidine independent for Salmonella typhimurium bacteria and tryptophan independent for Escherichia coli) were counted.
- COLONY COUNTING:
- Exposure duration: The revertant colonies (histidine independent/ tryptophan independent) were counted automatically with a Protos model 50000 colony counter or manually, if less than 40 colonies per plate were present. Plates with sufficient test article precipitate to interfere with automated colony counting were counted manually.
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- A test article is considered as mutagenic if in the strains TA 100, WP2, and its uvrA derivate the number of reversions will
be at least twice as high and in the strains TA 1535, TA 1537, and TA 98 it will be at least three times higher as compared to
the spontaneous reversion rate.
Also, a dose-dependent increase in the ntimber of revertants isregarded as an indication of possibly existing mutagenic potential
of the test article regardless whether the highest dose induced the above described enhancement factors or not.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium strains: TA100, TA98, TA1535, TA1537; E. coli : WP2uvrA and WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in strain TA 1535 at 5000 µg/plate without S9 mix in experiment I.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- PRECIPITATE: Not mentioned.
TOXICITY: Toxic effects evidenced by a reduction in revertant colony numbers occurred only in strain TA 1535 at 5000 µg/plate without S9 mix in experiment I. The plates incubated with the test article showed normal background growth up to 5000 µg/plate with and without metabolic activation in both independent experiments.
MUTAGENICITY: No increase in the number of revertants was observed upon treatment with the test substance under all conditions tested. All bacterial strains showed negative responses over the entire dose range, i.e. no dose-related, two-fold, increase in the number of revertants in two independently repeated experiments. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Number of revertants in the control or after treatment with the test substance
First experiment (10 - 5000 µg/plate) | |||||
Strain | Metabolic activation system | mean revertants in Controls | maximum revertant factor | dose dependency | Assessment |
TA 1535 | no | 15 | 1.1 | no | negative |
yes | 15 | 1.2 | no | negative | |
TA 1537 | no | 6 | 1.1 | no | negative |
yes | 6 | 1.4 | no | negative | |
TA 98 | no | 20 | 1.2 | no | negative |
yes | 33 | 1.2 | no | negative | |
TA 100 | no | 78 | 0.9 | no | negative |
yes | 88 | 1.1 | no | negative | |
E. coli WP2 uvrA | no | 38 | 1.2 | no | negative |
yes | 41 | 1.1 | no | negative | |
E. coli WP2 | noyes | 3040 | 1.61.2 | nono | negativenegative |
Second experiment (10 - 1000 µg/plate) | |||||
Strain | Metabolic activation system | mean revertants in Controls | maximum revertant factor | dose dependency | Assessment |
TA 1535 | no | 14 | 1.1 | no | negative |
yes | 15 | 1.2 | no | negative | |
TA 1537 | no | 11 | 1.2 | no | negative |
yes | 15 | 1.2 | no | negative | |
TA 98 | no | 24 | 1.2 | no | negative |
yes | 41 | 1.3 | no | negative | |
TA 100 | no | 90 | 1.1 | no | negative |
yes | 91 | 1.0 | no | negative | |
E. coli WP2 uvrA | no | 39 | 1.4 | no | negative |
yes | 47 | 1.1 | no | negative | |
E. coli WP2 | no | 57 | 1.1 | no | negative |
yes | 56 | 1.1 | no | negative | |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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