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EC number: 421-320-0 | CAS number: 118289-55-7 CP-89,575
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 April - 16 May 1996
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 92/69/EEC, Method C4-E
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Principles of method if other than guideline:
- Sealed bottles containing the test substance and inorganic mineral medium were inoculated with activated sewage sludge bacteria and incubated for up to 28 days at 20 ± 1°C. On Days 0, 4, 7, 11, 14, 18, 21, 25 and 28 duplicate bottles were removed and dissolved oxygen measurements were performed electrochemically. Percentage biodegradation values were calculated by comparing the
extent of oxygen depletion with the Theoretical Oxygen Demand (ThODN03 = 1.74 mg02/mg). Additional bottles, containing both the test substance and a readily biodegradable standard substance were prepared in order to provide additional information on the inhibitory effect of the test substance (Days 0, 14 and 28 only - GLP compliance:
- yes
Test material
- Reference substance name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- EC Number:
- 421-320-0
- EC Name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- Cas Number:
- 118289-55-7
- Molecular formula:
- Hill formula: C10 H9 Cl2 N O CAS formula: C10 H9 Cl2 N O
- IUPAC Name:
- 6-chloro-5-(2-chloroethyl)-2,3-dihydro-1H-indol-2-one
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Use: Pharmaceutical intermediate
Date recieved: 2 February 1996
Purity: 99.9%
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
STANDARD SUBSTANCE
Sodium benzoate (C6H5.COONa).
Fisons A.R. Grade, Batch No. 9401096045, Purity >99.5%.
Study design
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- Type: Activated sewage sludge bacteria.
Source: The aeration stage of the Anglian Water sewage treatment plant (Godmanchester) treating predominantly domestic sewage
Preparation: A sample was collected on 17 April 1996 and maintained under aeration for < 24 hours ,allowed to settle for 30 minutes and the supernatant filtered through Whatman GFA coarse filter paper (first 250 ml discarded). The filtrate was maintained under aeration until used. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- Dissolved oxygen
- Details on study design:
- Biochemical Oxygen Demand bottles (280 ml) of darkened glass and fitted with ground glass stoppers were filled by siphon and firmly stoppered to exclude all air bubbles. Sufficient bottles were prepared in each test and control series to allow two bottles to be removed at each time point for a single oxygen determination per bottle to be made at 0, 4, 7, 11, 14, 18, 21, 25 and 28 days. Sufficient bottles for series (d) were prepared to provide information on inhibitory effects on Days 0, 14 and 28 only.
The bottles were incubated in a water bath at 20 ± 1°C until removed for measurement of dissolved oxygen concentration by means of a Yellow Springs BOD Meter
Reference substance
- Reference substance:
- other: Sodium benzoate
Results and discussion
- Preliminary study:
- N/A
- Test performance:
- Cultures containing both test and standard substances combined showed an oxygen depletion value equivalent to 40 % biodegradation based on the combined theoretical oxygen demands of the test and standard substances (1.74 and 1.67 mgO/mg respectively). Consequently CP-89,575 was not considered to have had an inhibitory effect on sewage bacteria under the conditions of this test.
Sodium benzoate attained 99% biodegradation within 28 days.
Oxygen depletions in the inoculated control series were within the prescribed limits ( 1.5 mgO/l after 28 days).
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 20
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
5 % degradation after 4 d
7 % degradation after 7 d
5 % degradation after 11 d
6 % degradation after 14 d
6 % degradation after 18 d
12 % degradation after 21 d
0 % degradation after 25 d
20 % degradation after 28 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
90 % degradation after 4 d
80 % degradation after 7 d
82 % degradation after 11 d
78 % degradation after 14 d
97 % degradation after 18 d
99 % degradation after 21 d
91 % degradation after 25 d
99 % degradation after 28 d
Any other information on results incl. tables
Culturemedium |
Day |
|||||||||
0 |
4 |
7 |
11 |
14 |
18 |
21 |
25 |
28 |
||
(a) Mineralmediumwithinnculum
(b) Testsubstance(2mg/I)
(c) Standardsubst3ncc(3m/l)Sodiumbenzoatc --
(d)Testsubsta1K'C{21110:/IJplusstandardsubstance(3 m/l) |
R1R2 Mean
R1 R2 Mean
R1 R,
RIR, Mean |
9.030 9.030 9.030
9.030 9.030 9.030
9.030 9.030 9.030
9.030 9.030 9.030 |
9.010 8.820 8.915
8.630 8.820 8.725
4.820 3.970 4.395 |
8.540 8.650 8.595
8.320 8.400 8.360
4.62 4.53 4.575 |
8.340 8.210 8.275
8.260 7.970 8.115
4.280 4.090 4.185 |
8.330 7.910 8.120
7.910 7.890 7.900
4.340 4.100 4.2'.W
4.500 5.000 4.750 |
7.770 7.950 7.860
7.540 7.770 7.655
3.050 2.980 3.015 |
7.830 8.010 7.920
7.440 7.570 7.505
3.070 2.880 2.975 |
7.680 7.300 7.490
7.540 7.510 7.525
2.900 2.960 2.930 |
7.700 7.750 7.725
7.090 6.960 7.025
2.710 2.860 2.785
3.840 3.600 3.720 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- CP-89,575 may not be termed as readily biodegradable.
There was no evidence of inhibitory effects on the activity of the activated sewage sludge under the conditions of this test.
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